建立同类提取法从基因芯片检测数据中挖掘大鼠肝细胞的肝再生关键基因  

Establishing Same Kind Extraction Method to Find the Hepatocyte Key Genes in Rat Liver Regeneration from the Data of Gene Microarray

作　　者：徐存拴 闫春玲[1,2] 江云 周运 黄荧[3] 李钧涛[3] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]省部共建细胞分化调控国家重点实验室培育基地,河南新乡453007 [3]河南师范大学数学与信息科学学院,河南新乡453007

出　　处：《河南科学》2013年第6期762-767,共6页Henan Science

基　　金：国家973项目前期研究专项基金资助项目(2010CB534905)

摘　　要：为解析基因芯片检测数据的生物学意义,用Rat Genome 230 2.0芯片检测大鼠肝再生中肝细胞的基因表达丰度,用F检验大鼠2/3肝切除组(PH)与假手术组(SO)的基因表达差异性和获得大鼠肝细胞的肝再生相关基因,用同类提取法从过滤法计算的差异基因中筛选特征基因,根据特征基因的关联度、参与的生理活动和他人研究结果确认其中的关键基因.结果表明,Ccne1、Egf、Met等57个特征基因在大鼠肝再生中起关键作用.To highlight the biological significance of gene microarray data,Rat Genome 230 2.0 Arrays were used to detect expression abundance of the hepatocyte genes in rat liver regeneration,F-test to test the gene expression differences and to identify the liver regeneration associated-genes in rat two-thirds hepatectomy（PH）and sham operation（SO）,same kind extraction method to select the hepatocyte feature genes in rat liver regeneration,which came from the differential genes evaluated by Filter method,then the liver regeneration key genes of rat hepatocytes were identified according to their connectivities,physiological activities and research results.The research shows that Ccne1,Egf,Met,etc 57 feature genes played key roles in the rat liver regeneration.

关 键 词：大鼠肝再生 过滤法 同类提取法 差异基因 特征基因 关键基因 

分 类 号：Q332[生物学—遗传学]