不同温度下CO_2浓度增高对坛紫菜生长和叶绿素荧光特性的影响  

The combined effects of increasing CO_2 concentrations and different temperatures on the growth and chlorophyll fluorescence in Porphyra haitanensis(Bangiales,Rhodophyta)

作　　者：刘露[1] 丁柳丽[1] 陈伟洲[2] 邹定辉[1] 

机构地区：[1]华南理工大学环境与能源学院,广州510006 [2]汕头大学海洋生物研究所,汕头515063

出　　处：《生态学报》2013年第13期3916-3924,共9页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(41076094;41276148)

摘　　要：大气CO2浓度升高对海藻的影响已有许多的研究报道,但鲜见有关温度与CO2相互作用的研究。在4种条件下对坛紫菜进行连续通气培养:(1)15℃+390μmol/mol CO2,(2)15℃+700μmol/mol CO2,(3)25℃+390μmol/mol CO2,(4)25℃+700μmol/mol CO2。从而探讨这种南方海域重要栽培海藻种类的生长和叶绿素荧光特性对温度和CO2相互作用的响应。结果表明:CO2对坛紫菜的生长的影响具有温度依赖性,在低温生长条件下提高CO2浓度更有利于坛紫菜的生长。CO2对坛紫菜叶绿素a(Chlorophyll a,Chl a)和类胡萝卜素(Carotenoid,Car)的促进作用远大于温度对其产生的影响。相对于25℃的生长温度而言,15℃生长温度下的坛紫菜表现出较高的最大相对电子传递速率(rETRmax),表明坛紫菜在低温环境下有较高的光合潜力;而CO2对坛紫菜的rETRmax没有明显影响。对于在不同测定温度下的光合荧光特性而言,在10—30℃测定温度范围内,在各生长条件下的海藻的rETRmax、光能利用效率(α)和最大光化学量子产量(Fv/Fm)随温度的升高变化不明显;但在较高测定温度下(≥30℃),上述荧光参数显著下降,说明高温易引发海藻光能利用效率和光合能力的下降,这可能与光系统(PS)Ⅱ反应中心活性下调有关。同时,当测定温度大于30℃时,15℃生长条件下的坛紫菜的rETRmax、α和Fv/Fm值下降趋势远大于25℃生长条件下的坛紫菜的值,表明在低温生长条件下的坛紫菜对短期高温胁迫的适应能力较弱;而在高CO2浓度生长条件下的坛紫菜的rETRmax总是低于正常CO2浓度生长下的值,说明CO2浓度升高会抑制坛紫菜在短期高温条件下的光合电子传递能力。Atmospheric CO 2 concentration is predicted to nearly double by the end of this century.There are a large number of reports on the effects of elevated atmospheric CO 2 concentrations on seaweeds.However,the investigation concerning the impacts of combined effects of elevated atmospheric CO 2 concentrations and temperature on seaweeds is very limited.In the present study,the red seaweed Porphyra haitanensis（Bangiales,Rhodophyta） was cultured under four different conditions：（1） 15 ℃ ＋ 390μmol/mol CO 2,（2） 15 ℃ ＋ 700μmol/mol CO 2,（3） 25 ℃ ＋ 390μmol/mol CO 2,（4） 25 ℃ ＋ 700μmol /mol CO 2,in order to examine the combined effects of the elevated CO 2 concentrations and temperature on growth,some biochemical components and chlorophyll fluorescence in this commercially important species.The results indicated that the growth response of P.haitanensis to elevated CO 2 in seawater was temperature dependent,with the enhancement of growth resulting from elevated CO 2 being remarkable greater at lower growth temperature（15 ℃） with respect to 25 ℃.The CO 2 conditions in culture exerted a much pronounced influence on the contents of Chlorophyll a（Chl a） and Carotenoid（Car） than temperature did.15 ℃-grown P.haitanensis exhibited much higher maximum relative electron transport rate（rETR max） in comparison with 25 ℃-grown thalli,regardless of the CO 2 concentrations in culture.This indicated that P.haitanensis displayed a higher photosynthetic potential at low growth temperature than high growth temperature.Meanwhile,extra CO 2 in the culture had no significant impact on rETR max in P.haitanensis.The characters of chlorophyll fluorescence were determined under different measurement temperatures using Junior-PAM.The increase of measurement temperature from 10 to 30 ℃ made a negligible effect on rETR max,the photosynthetic light use efficiency of electron transport（α,the initial slope of the rapid light curves） and maximum photochemical quantum yield values（F v

关 键 词：CO2 温度 坛紫菜 生长 叶绿素荧光 光合作用 

分 类 号：Q948.8[生物学—植物学]