手术治疗对鼻-鼻窦恶性肿瘤患者辅助性T细胞的影响  

Effects of surgical operation on helper-inducer T-lymphocytes in sino-nasal neoplasms patients

作　　者：孙洁[1] 刘勤松[1] 李敬华[2] 袁勇[1] 薛卫国[1] 

机构地区：[1]青岛市市立医院耳鼻咽喉科,266011 [2]青岛市市立医院中心实验室,266011

出　　处：《中华医学杂志》2013年第26期2065-2067,共3页National Medical Journal of China

摘　　要：目的探讨手术治疗对鼻-鼻窦恶性肿瘤辅助性T细胞（Th）1／Th2的影响。方法2004年1月至2011年12月，选取青岛市市立医院耳鼻咽喉科80例鼻-鼻窦恶性肿瘤患者为试验组，80例鼻中隔偏曲患者为对照组，用流式细胞仪测定试验组与对照组术前、术后的外周血单个核细胞，经佛波酯诱导培养后测定Th1内干扰素（IFN）γ和Th2内白细胞介素（IL）4的水平。结果术前试验组CD3＋CD8-IL4＋细胞水平高于对照组（6．6％±1．7％比2．8％±1．7％，P〈0．05），而CD3＋CD8-IFN-γ细胞水平低于对照组（18．7％±5．7％比59．3％±1．5％，P〈0．05）。试验组术后3个月外周血CD3＋CD8-IL4＋细胞水平低于术前（2．8％±1．5％比6．6％±1．7％，P〈0．05），而CD3＋CD8-IFN-γ细胞水平高于术前（54．0％±4．0％比18．7％±5．7％，P〈0．05）。结论手术治疗可促使鼻．鼻窦恶性肿瘤患者外周血Th1／Th2免疫恢复平衡。Objective To explore the effects of surgical operation on helper-inducer T-lymphocytes （Th1/Th2） in sino-nasal neoplasms. Methods From January 2004 to December 2011, 80 patients with malignant tumor in nasal cavities or sinuses were enrolled as experimental group and another 80 subjects with deflection of nasal septum as control group. The phorbol-12-myristate-13-acetate （PMA）-stimulated peripheral blood mononuclear cells （PBMC） were obtained from both malignant tumor and normal control tissues. And flow cytometry was used to detect the expression percentages of interleukin-4 （IL-4） in Th2 cell and interferon-gamma （IFN-＇y） in Thl cell at pre and post-operation. Results The preoperative expression percentage of CD3 ＋ CD8 - IL-4 ＋ cell in malignant tumor patients was higher than that in controls （6. 6% ± 1.7% vs 2. 8% ± 1.7% ,P 〈0. 05） while the expression percentage of CD3 ＋ CD8 - IFN-γ cell was lower （ 18.7% ± 5.7% vs 59.3% ± 1.5% ,P 〈 0. 05 ）. The preoperative expression percentage of CD3 ＋ CD8 - IL-4 ＋ cell was higher than that at postoperation （6.6% ± 1.7 %vs 2. 8% ± 1.5%, P 〈0.05）.And the postoperative expression percentage of CD3 ＋ CD8 - IFN-γ cell was higher than that at preoperation （54. 0% ± 4. 0% vs 18. 7% ±5.7% , P 〈 0.05 ）. Conclusion Surgery may restore the immune balance in patients with malignant tumors in nasal cavities or sinuses.

关 键 词：流式细胞术 鼻窦肿瘤 T淋巴细胞 辅助诱导 白细胞介素4 干扰素Ⅱ型 

分 类 号：R739.62[医药卫生—肿瘤]