γ突触核蛋白与基质金属蛋白酶9在乳腺癌中表达的相关性  

The correlation of synuclein-γ and matrix metalloproteinase 9 in breast cancer

作　　者：陈健[1] 黄硕[1] 吴克瑾[1] 王永坤[1] 贾谊君[1] 陆云姝[1] 翁子毅[1] 

机构地区：[1]上海交通大学医学院附属新华医院普外科,200092

出　　处：《中华外科杂志》2013年第7期641-644,共4页Chinese Journal of Surgery

摘　　要：目的 检测γ突触核蛋白（SNCG）与基质金属蛋白酶9（MMP-9）在浸润性乳腺癌组织以及T47D和T47 DSNCG-细胞中的表达,探讨SNCG与MMP-9的相关性.方法 选择2009年6月至2012年6月手术切除的96例浸润性乳腺癌标本[女性,年龄（56±8）岁],采用免疫组化法分别检测组织标本中SNCG和MMP-9的表达情况.建立T47D与SNCG干扰T47DSNCG-细胞株,采用Western blot法检测SNCG与MMP-9蛋白的表达,real-time PCR检测SNCG与MMP-9基因的表达.结果 96例标本中,26例（27.1％） SNCG与MMP-9同时表达,30例（31.12％）同时不表达,二者之间的表达呈明显正相关（r =0.655,P=0.000）.T47D细胞株SNCG蛋白表达水平是T47DSNCG-细胞株的2.1倍,SNCG mRNA表达水平约为T47DSNCG-细胞的13.5倍.T47D细胞株MMP-9蛋白表达水平是T47DSNCG-细胞株的1.6倍,MMP-9 mRNA表达水平是T47DSNCG-细胞株的7.3倍.结论 SNCG、MMP-9蛋白在乳腺癌中的表达呈明显正相关.SNCG可能通过调节MMP-9的表达而影响乳腺癌的浸润、转移.Objectives To evaluate the expression of synuclein-γ （SNCG） and metalloproteinase 9 （MMP-9） both in the invasive ductal breast cancer samples and T47D and T47D^SNCG- cell lines, to investigate the correlation between SNCG and MMP-9. Methods Totally 96 invasive ductal breast cancer samples （female, mean age of （56 ± 8） years） were collected between June 2009 and June 2012. The expressions of SNCG and MMP-9 were investigated by immunohistochemistry. T47D and SNCG knock down T47DsNcG- cell lines were established and SNCG and MMP-9 protein expression were investigated by Western blot and gene expression by real-time PCR. Results Among 96 samples, 26 （27. 1% ） of them co-expressed SNCG and MMP-9, 30（31.2% ） of them expressed neither SNCG nor MMP-9. The expression of SNCG was correlated with the expression of MMP-9（r =0. 655, P =0. 000）. SNCG mRNA level of T47D cell line was 13.5 fold of T47DSNCG- cell line and SNCG protein expression was 2. 1 fold. While MMP-9 mRNA level of T47D cell line was 7. 3 fold of T47DSNCG- cell line and MMP-9 protien expression was 1.6 fold. When SNCG was knocked down, the expression of MMP-9 decreased. Conclusions SNCG and MMP-9 are significantly correlated with each other in breast cancer. SNCG may promote the invasion and metastasis of breast cancer mediated by up-regulating the expression of MMP-9.

关 键 词：癌 导管 乳腺 γ突触核蛋白 基质金属蛋白酶9 

分 类 号：R737[医药卫生—肿瘤]