Lung microRNA profile in chronic cyanotic piglets with decreased pulmonary blood flow  

作　　者：WANG Dong LIU Ying-long LU Xiao-dong LING Feng LIU Ai-jun DU Jie HAN Ling 

机构地区：[1]Department of Pediatric Cardiac Center Beijing Anzhen Hospital, Capital Medical University, Beijng 100029, China [2]Institute of Heart Lung and Blood Vessel Diseases Beijing Anzhen Hospital, Capital Medical University, Beijng 100029, China [3]Beijing Anzhen Hospital, Capital Medical University, Beijng 100029, China Department of Pediatric Cardiac Surgery Center, Fuwai Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100037, China [4]Department of Cardiothoracic Surgery, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, China

出　　处：《Chinese Medical Journal》2013年第12期2260-2264,共5页中华医学杂志（英文版）

基　　金：This study was supported by grants from the National Natural Science Foundation of China （No. 81100116）, the Beijing Natural Science Foundation （No. 7112046）, and Capital Citizens＇ Health Project Cultivation of Beijing Municipal Science and Technology Commissions （No. Z 111100074911001）.Acknowledgments： The authors are grateful to Dr. LI Gang and Dr. XU Yu-lin for the animal experiment＇s assistance, and ZHANG Yanbin and ZHANG Shao-bin for their bioinformatics assistance.

摘　　要：Background Cyanotic congenital heart defects with decreased pulmonary blood flow due to lung ischemia,hypoxia,and others lead to infant morbidity and mortality more than acyanotic heart disease does.Despite the great effort of medical research,their genetic link and underlying microRNAs molecular mechanisms remain obscure.In this study,we aimed to investigate microRNAs regulation during cyanotic defects in lung of immature piglets.Methods Cyanotic piglet model was induced by main pulmonary artery-left atrium shunt with distal pulmonary artery banding.Four weeks later,hemodynamic parameters confirmed the development of cyanotic defects and pulmonary lobe RNA was extracted from all animals.We studied the repertoire of porcine lung microRNAs by Solexa deep sequencing technology and quantified highly expressed microRNAs by microarray hybridization.Furthermore,we quantitated selected microRNAs from cyanotic and control piglets by quantitative RT-PCR.Results After surgical procedure 4 weeks later,the cyanotic model produced lower arterial oxygen tension,arterial oxygen saturation,and higher arterial carbon dioxide tension,hematocrit and hemoglobin concentration than controls （all P 〈0.05）.In 1273 miRNAs expressed in the immature piglets lungs,2 most abundant microRNAs （miR-370 and miR-320） demonstrated significant difference between cyanotic and control group （all P 〈0.05）.Conclusion Our results extended lung microRNA profile in immature piglets and suggested that miR-370 and miR-320 are significantly up-regulated in cyanotic lung tissues.Background Cyanotic congenital heart defects with decreased pulmonary blood flow due to lung ischemia,hypoxia,and others lead to infant morbidity and mortality more than acyanotic heart disease does.Despite the great effort of medical research,their genetic link and underlying microRNAs molecular mechanisms remain obscure.In this study,we aimed to investigate microRNAs regulation during cyanotic defects in lung of immature piglets.Methods Cyanotic piglet model was induced by main pulmonary artery-left atrium shunt with distal pulmonary artery banding.Four weeks later,hemodynamic parameters confirmed the development of cyanotic defects and pulmonary lobe RNA was extracted from all animals.We studied the repertoire of porcine lung microRNAs by Solexa deep sequencing technology and quantified highly expressed microRNAs by microarray hybridization.Furthermore,we quantitated selected microRNAs from cyanotic and control piglets by quantitative RT-PCR.Results After surgical procedure 4 weeks later,the cyanotic model produced lower arterial oxygen tension,arterial oxygen saturation,and higher arterial carbon dioxide tension,hematocrit and hemoglobin concentration than controls （all P 〈0.05）.In 1273 miRNAs expressed in the immature piglets lungs,2 most abundant microRNAs （miR-370 and miR-320） demonstrated significant difference between cyanotic and control group （all P 〈0.05）.Conclusion Our results extended lung microRNA profile in immature piglets and suggested that miR-370 and miR-320 are significantly up-regulated in cyanotic lung tissues.

关 键 词：microRNAs sus scrofa LUNG CYANOSIS microarray analysis 

分 类 号：R-332[医药卫生]