云芝多糖-B对人食管癌细胞Eca109转移的影响  

Effects of CVPs-B on Metastatic Characteristics of Human Esophageal Carcinoma Cell Line Eca109

作　　者：汪道峰[1] 李小东[2] 娄宁[1] 

机构地区：[1]华南肿瘤学国家重点实验室//中山大学肿瘤防治中心ICU,广东广州510060 [2]华南肿瘤学国家重点实验室//中山大学肿瘤防治中心胸科,广东广州510060

出　　处：《中山大学学报（医学科学版）》2013年第3期339-344,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金(S2011040004502);广东省科技计划项目(2010B030700051)

摘　　要：【目的】应用新型云芝多糖-B(CVPs-B)作用食管鳞癌Eca109细胞,探讨其对Eca109细胞转移特性的作用。【方法】Western blot检测CVPS-B作用后,食管鳞癌Eca109细胞CXCL12蛋白表达的变化,Transwell侵袭实验评估食管癌细胞侵袭能力的变化,MTT法观察食管癌细胞与Matrigel胶黏附能力的变化,划痕法检测食管癌细胞迁移能力的变化。【结果】实验组Eca109细胞CXCL12蛋白表达水平较对照组显著降低(P<0.05)。CVPs-B孵育后,食管癌Eca109细胞黏附、侵袭及迁移能力下降。【结论】CVPs-B降低食管癌细胞CXCL12蛋白表达,有效抑制食管癌Eca109细胞的转移潜能,提示CVPs-B可能可以通过CXCL12/CXCR4通路抑制食管鳞癌细胞转移特性。[Objective] The aim of this study was to explore the effects of Coriolus versicolor polysaccharide-B （CVPs-B） on the metastatic characteristics of human esophageal carcinoma cell line Eca109 in vitro.[Methods] The expression of CXCL12 in the cell line Eca109 was detected by Western blot.The adhesive ability of Eca109 cells to extracellular matrix matrigel was evaluated by MTT assay.The invasive ability of Eca109 cells was measured by Transwell experiment.The locomotion ability of Eca109 cells was measured by scrape assay.[Results] Compared with control group,CXCL12 protein expression in experimental group was downregulated （P 〈 0.05）.Meanwhile,the number of Eca 109 cells infiltrated Transwell membrane in experimental group was decreased （P〈 0.05）.The adhesion rate of experimental group cells was significantly reduced （P 〈 0.05）.The locomotion ability of experimental group cells was reduced.[Conclusion] CVPs-B can reduced expression of CXCL12 protein in esophageal carcinoma cells.It can inhibit metastatic potential of Eca109 cells.CVPs-B maybe inhibit the transfer characteristics of squamous cell carcinoma of the esophagus through the CXCL12/CXCR4 pathway.

关 键 词：食管癌 CVPs—B CXCL12 CXCR4 转移 

分 类 号：R734.1[医药卫生—肿瘤]