新型多醛基海藻酸钠-肝素复合涂层的制备和筛选  

Preparation and selection of a new sodium alginate-heparin composite coating with multi-aldehyde groups

作　　者：高文卿[1,2] 李彤[1] 于美丽[3] 胡晓旻[1] 段大为[1] 许晋[1] 

机构地区：[1]天津市第三中心医院,天津市300170 [2]天津医科大学研究生院,天津市300070 [3]天津市人工细胞重点实验室,天津市300170

出　　处：《中国组织工程研究》2013年第25期4668-4675,共8页Chinese Journal of Tissue Engineering Research

基　　金：天津市科技支撑计划重点项目(11ZCGYSY02000);天津市科委一般项目(09JCYBJC27600)~~

摘　　要：背景:前期实验证明多醛基海藻酸钠-肝素复合涂层具有良好生物相容性和血液相容性,但对于最优制备条件和最优模式尚不清楚。目的:研制一种新型多醛基氧化海藻酸钠-肝素复合涂层,筛选新型涂层制备的最佳条件。方法:以正交实验筛选氧化海藻酸钠(或肝素)最优涂层条件,获得3种氧化海藻酸钠/肝素复合涂层模式,将复合涂层通过共价交联的方式固定到氨基化预处理/化学修饰的聚氯乙烯管道表面,从涂层物定量、蛋白黏附实验和表面接触角实验3个方面筛选最优复合涂层模式,并检测最优复合涂层的抗凝血性能。结果与结论:氧化海藻酸钠涂层物最优涂层条件为50%浓硫酸、0.05%聚乙烯亚胺、反应溶液pH值3.5、反应温度为40℃和氧化海藻酸钠质量浓度为2g/L。肝素涂层物最优涂层条件为70%浓硫酸、0.1%聚乙烯亚胺、反应溶液pH值3.5、反应温度为30℃和肝素质量浓度为0.1g/L。复合涂层物最佳涂层模式氧化海藻酸钠、肝素涂层量分别为(4.07±1.35),(2.13±0.24)μg/cm2,此复合涂层具有良好的生物相容性及抗凝血性能。BACKGROUND： Preliminary experiments have demonstrated that composite coating of sodium alginate and heparin with multi-aldehyde groups has excellent biocompatibility and hemocompatibility. However, little is know about the optimal condition and pattern of new coating preparation. OBJECTIVE： To prepare a new composite coating of sodium alginate and heparin, and to optimize the coating preparation conditions. METHODS： The optimal coating conditions of sodium alginate （or heparin） were screened by orthogonal experiment, to obtain three coating patterns of sodium alginate/heparin composite. Composite coating was covalently bonded on the polyvinyl chloride surface which was preprocessed by amination/chemical modification The screening methods of optimal composite coating pattern included coating quantification, protein adhesion test and surface contact angle. Anticoagulation properties of optimum composite coating model were assessed. RESULTS AND CONCLUSION： The optimum coating condition of oxidated sodium alginate in composite coating was 50% concentrated sulphudc acid, 0.05% polyethyleneimine, pH 3.5, reaction temperature 40℃ and 2 g/L oxidated sodium alginate. The optimum coating condition of heparin in composite coating was 70% concentrated sulphuric acid, 0.1% polyethyleneimine, pH 3.5, reaction temperature 30 ℃ and 0.1 g/L low molecular nitrous acid-converted heparin. The quantities of oxidated sodium alginate and heparin in optimum composite coating pattern were （4.07±1.35） and （2.13±0.24） μg/cm2. And the composite coating possesses fine anticoagulant property and biocompatibility.

关 键 词：生物材料 组织工程复合支架材料 海藻酸钠 肝素 复合涂层 共价结合 氧化 重氮化 生物相容性 抗凝血性能 省级基金 

分 类 号：R318[医药卫生—生物医学工程]