胃癌组织的表面增强拉曼光谱研究  

Surface-Enhanced Raman Spectroscopy for Gastric Cancer and Normal Tissue

作　　者：巩龙静[1] 马君[1] 周涵婧[1] 刘澍[1] 周正华[1] 毛伟征[2] 郑荣儿[1] 

机构地区：[1]中国海洋大学光学光电子实验室,山东青岛266100 [2]青岛大学医学院附属青岛市立医院普外科,山东青岛266071

出　　处：《中国海洋大学学报（自然科学版）》2013年第7期99-103,共5页Periodical of Ocean University of China

基　　金：青岛市科技发展计划项目(08-1-3-31-jch)资助

摘　　要：利用表面增强拉曼光谱(SERS)对胃癌与正常组织进行识别研究。以金溶胶为基底,分别对胃癌及正常的组织、组织匀浆和上清液进行SERS探测,结合生物中几种常见物质的固体拉曼光谱,确定各特征峰的归属,并对比分析胃癌及正常组织SERS的区别。结果表明:金溶胶明显地增强了组织、匀浆和上清的拉曼信号,其中上清的增强效果最好;相比正常组织的SERS,癌组织在724、1 329、1 367和1 597cm-1处峰强度较高,在828和1 002cm-1处峰强度较低;相比正常组织匀浆上清的SERS,癌上清在623、724、1 087、1 471、1 597和1 723cm-1处峰强度较高,在828、1 002和1 032cm-1处峰强度较低;反映出癌组织核酸的含量较高,大部分蛋白质、糖类及脂类的含量较低。研究表明,胃组织的SERS信号比普通拉曼信号有明显的增强,胃癌组织与正常组织的SERS存在差异,有望成为胃癌临床诊断的一种新方法。The purpose of this study is to detect gastric tissues and to distinguish cancerous and normal tissues with surface-enhanced Raman spectroscopy （SERS）. The SERS spectra of tissues, homogenate and supernatant of homogenized tissues were measured in this experimentation. The results show that the enhancement effect, especially for supernatant of homogenized tissues, is obvious comparing with normal tissues, the gastric cancer tissues have higher Raman intensities at 724, 1 329, 1 367 and 1 597 cm-1, but lower at 828 and 1 002 cm-1 comparing with normal supernatant of homogenized tissues, the gastric cancer supernatant has higher Raman intensities at 623, 724, 1 087, 1 471, 1 597 and 1 723 cm-1 , but lower at 828, 1 002 and 1 032 cm-1. The results indicate that there are higher content of nucleic acides and lower content of most of proteins, carbohydrate and lipids in carcinoma tissues. This preliminary study demonstrates that it is possible to diagnose gastric cancer using SERS technology in the future.

关 键 词：光谱学 胃癌 表面增强拉曼光谱 离体组织 金溶胶 

分 类 号：Q631[生物学—生物物理学] R735.2[医药卫生—肿瘤]