人参皂苷Re致中国仓鼠肺细胞染色体畸变作用  

Effect of ginsenoside Re on chromosome aberration in Chinese hamster lung cells

作　　者：曹冲 高梅 杨麟珂[2] 张婷婷[2] 

机构地区：[1]山东省医药工业研究所山东省化学药物重点实验室,济南250101 [2]山东省肿瘤医院

出　　处：《医学动物防制》2013年第7期712-714,717,共4页Journal of Medical Pest Control

摘　　要：目的研究人参皂苷Re致中国仓鼠肺细胞(Chinese hamster lung cells,CHL)染色体畸变的作用。方法细胞计数法测定人参皂苷Re对CHL细胞的半数抑制浓度(50%inhibition concentration,IC50),根据IC50设立不同剂量组,进行染色体畸变试验,分别观察人参皂苷Re染毒6h、24 h及加S9后染毒6 h CHL细胞染色体的数目及结构变化,进行染色体畸变分析。结果人参皂苷Re染毒6h、24 h及加S9后染毒6 h CHL细胞染色体畸变为阴性。结论在本试验条件下,人参皂苷Re不能引起CHL细胞染色体产生畸变。Objective To explore the effect of ginsenoside Re on chromosome aberration in Chinese hamster lung （CHL） ceils. Methods Use the method of cell counting to determine the IC50 of ginsenoside Re on CHL cells, then to establish the range of doses according to the IC50 and to do the cell chromosome aberration experiment. When the CHL cells were exposed to ginsenoside Re at 6h and 24h and plus S9 mixture at 6h respectively, we observe the changes of chromosome number and structure, then to judge the chromosome aberration results. Results Negative response was found at 6h and 24 h after the treatment with ginsenoside Re and at 6h after the addition of S9 mixture. Conclusions Under the condition of this experiment, ginsenoside Re does not induce chromosome aberration in CHL cells.

关 键 词：人参皂苷RE 中国仓鼠肺细胞 半数抑制浓度 染色体畸变 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]