用三色荧光原位杂交检测人精子染色体非整倍体率  

Using the method of tripl-color fluorescence in situ hybridization to detect aneuploidy of human sperms

作　　者：郭虹[1] 王波[1] 

机构地区：[1]湖北省妇幼保健院检验科,430070

出　　处：《中国优生与遗传杂志》2013年第6期43-44,58,F0002,共4页Chinese Journal of Birth Health & Heredity

摘　　要：目的建立人精子间期核三色荧光原位杂交(tripl-color fluorescence in situhybr idizat ion)的实验方法。方法采用三色荧光原位杂交技术对10例和正常精子标本进行间期核的原位杂交,并统计精子间期核13、16、18、21、22、X、Y染色体的杂交信号数量。结果在显微镜下可见精子头部有杂交探针的信号,间期核背景经DAPI复染显示蓝色。结论本法具有荧光杂交信号直观易分辨、短时间内能大量分析精子数量和实验操作相对简便、结果准确可信等优点。Objective：To establish an experimental approach tripl-color fluorescence in situ hybridization of human sperm interphase nucleus.Methods：The technique of tripl-color fluorscence in situ hybridization was used in 10 cases of normal sperm specimens,and the number of hybridization signals for 13,16,18,21,22,X and Y chromosome in the sperm interphase nucleus were counted.Results：It was seen under the microscope that the probe showed hybridization signal.Interphase nucleus counter stained with DAPI showed blue.Conclusions：The merits of this method are the hybridization signals visible and distinguishable,analyzing the quantit y of sperm on a large scale,simple performance of experiments and accurate and reliable results.

关 键 词：三色荧光原位杂交 非整倍体率 人精子染色体 

分 类 号：R394.3[医药卫生—医学遗传学]