活络效灵丹中四种有效成分提取工艺研究  

Research on Extraction Technology of Four Effective Constituents from "Huoluo Xiaoling Pill"

作　　者：韩向东[1] 黄云枫[1] 张彤[1] 陈萍[1] 邱水平[1] 邵亚楠[1] 

机构地区：[1]上海中医药大学中药学院,上海201203

出　　处：《上海中医药大学学报》2013年第3期105-109,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81273657);上海市人才发展基金项目(20111015)

摘　　要：目的:以阿魏酸、藁本内酯、丹酚酸B及丹参酮ⅡA四种有效成分为指标,优选活络效灵丹的提取工艺。方法:采用高效液相法测定当归中阿魏酸、藁本内酯及丹参中丹酚酸B、丹参酮ⅡA的含量,用正交试验方法综合评分优选复方提取工艺条件。结果:建立了同时测定当归中的两种有效成分阿魏酸、藁本内酯的方法,以及同时测定丹参中的两种有效成分丹酚酸B、丹参酮ⅡA的方法。经正交试验方法综合评分优选出最佳提取工艺为加8倍量、50%乙醇、提取时间0.5 h、提取3次。结论:正交试验优选出的最佳提取工艺为加8倍量50%乙醇、提取时间0.5 h、提取3次,按上述工艺将当归及丹参合并提取,该工艺简便易行,重现性好,四种有效成分的提取率均达到80%以上。Objective： To optimize the extraction technology of four effective constituents： ferulic acid,ligustilide,salvianolic acid B and tanshinone ⅡA from ＂Huoluo Xiaoling Pill＂.Methods： The HPLC method was used to determine the content of ferulic acid and ligustilide in Radix Angelicae Sinensis and salvianolic acid B and tanshinone ⅡA in Radix Salviae Miltiorrhizae.The extraction technology was evaluated by orthogonal design.Results： The ferulic acid and ligustilide in Radix Angelicae Sinensis and salvianolic acid B and tanshinone ⅡA in Radix Salviae Miltiorrhizae was determined.The optimum extraction condition as follows： extracted for 0.5h with 50% ethanol,extracted for 3 times.Conclusion： The result of the experiment showed the optimum extraction condition as follows： extracted for 0.5 hours with 50% ethanol 8 ml/g,decocting 3 times.The process to extract the four active principle in Chinese Angelica and Salvia Miltiorrhiza is viable and stable.The extraction rate of four active principles are more than 80%.

关 键 词：活络效灵丹 阿魏酸 藁本内酯 丹酚酸B 丹参酮ⅡA 提取工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学]