新型生物处理技术对干酪乳杆菌KL1胆盐水解酶活力及活菌数量的影响  

Effect of a New Biological Treatment Technology on the Activity of Bile Salt Hydrolase and Viable Count of Lactobacillus Casei KL1

作　　者：庞东辉[1] 李升平[1] 周妍[2] 刘慧[2] 

机构地区：[1]北京城市学院,北京100083 [2]北京农学院,北京102206

出　　处：《北京城市学院学报》2013年第3期67-71,共5页Journal of Beijing City University

基　　金：北京市自然科学基金资助项目(项目编号:5062006)

摘　　要：本文利用从藏灵菇中筛选的产胆盐水解酶的干酪乳杆菌KL1(Lactobacillus casei KL1)菌株,首次研究采用新型生物处理技术对胆盐水解酶(BSH)活力及活菌数量的影响规律。所谓新型生物处理技术,即运用人体生物能量对生物细胞及遗传物质产生影响,从而导致生物变异的技术,这是一项有别于现有的物理、化学因子的新型诱变技术。以茚三酮显色法测定胆盐水解酶反应液中氨基酸的吸光度(A570nm),根据A570nm大小分析新型生物处理技术对胆盐水解酶活力的影响;以紫外分光光度计测定培养液的光密度(OD600nm),根据OD600nm大小分析新型生物处理技术对KL1活菌数量的影响。结果表明,经新型生物处理技术处理后,干酪乳杆菌KL1胆盐水解酶活力有显著提高(p<0.05),其活菌数量有极显著提高(p<0.01)。显示新型生物处理技术对干酪乳杆菌KL1菌株胆盐水解酶活力及活菌数量影响显著,且干酪乳杆菌的活菌数量与其胆盐水解酶活力呈正相关。This research has studied the effect of a new biological treatment technology on the activity of ＂bile salt hydrolase （BSH） and viable count. The BSH was produced by Lactobacillus casei KL1 strain which separated from Tibetan Kefir. The new biological treatment technology is a method which leads to bio- mutation by using the effect of human biological energy on cells and genetic material. It is a new mutagene- sis technology, different from physical and chemical mutagenesis. At the first, the ab-sorbance of amino acid existing in BSH （at A570nm） was detected by ninhydrin reaction. Then the effect of the new biological treatment technology on the activity of BSH was determined. Secondly, the optical density （OD 600nm） of the broth was studied by UV spectrometer. Finally the effect of the new method on KL1 strain viable count was analyzed according to OD 600nm. The results showed that after handled by the new method, the activity of BSH was significant increased （ p 〈 0. 05 ）, as well as the viable count （ p 〈 0. 01 ）. It indicated that this new method can evidently increase the activity of BSH and viable count of KL1 strain, and the viable count and the activity BSH were in positive corrdation.

关 键 词：新型生物处理技术 干酪乳杆菌 胆盐水解酶 活菌数量 

分 类 号：TS252[轻工技术与工程—农产品加工及贮藏工程]