影响甲胎蛋白水平的候选单核苷酸多态性位点在肝癌细胞株中的验证  

Validation of candidate single nucleotide polymorphisms associated with α-fetoprotein levels in human hepatoma cell lines

作　　者：黄健旅[1] 梁毓[1] 刘征涛[1] 罗长志[1] 于龙[1] 王东阳[1] 李源[1] 彭涛[2] 

机构地区：[1]广西医科大学研究生学院,南宁530021 [2]广西医科大学第一附属医院肝胆外科

出　　处：《中国癌症防治杂志》2013年第2期95-99,共5页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：国家自然科学基金资助项目(NSFC81072321);2009年教育部新世纪优秀人才课题(02303003003);广西卫生厅重点科研课题(重201018)

摘　　要：目的检测坐落于甲胎蛋白(α-fetoprotein,AFP)基因候选单核苷酸多态性(single nucleotide polymorphisms,SNPs)位点rs12506899、rs16849384和位于白蛋白(albumin,ALB)基因候选SNP位点rs7688274基因型在肝癌细胞株中的分布及其对肝癌细胞株AFP水平的影响。方法选取AFP表达阴性的永生化正常肝细胞株HL-7702及AFP表达阳性的肝癌细胞株BEL-7402、BEL-7404、Hep-G2、MHCC-97H、SMMC-7721进行培养,检测其培养液中AFP的水平,提取细胞DNA,以Taq-manMGB探针法进行基因分型,对各组基因型间AFP的水平进行比较。结果 rs16849384T/T基因型的Hep-G2的AFP水平显著高于C/T基因型的SMMC-7721、HL-7702和BEL-7402(P分别为<0.001、0.001和0.015)。结论①坐落于AFP基因的rs16849384基因型与肝癌细胞株的AFP水平相关;②在肝癌细胞株中,位点rs16849384等位基因T为高值等位基因。Objective To explore the influences of the rs 12506899 and rs 16849384 polymorphisms in the ct-fetoprotein （AFP） gene and the rs 7688274 polymorphism in the albumin（ALB） gene on AFP levels in human hepatoma cell lines. Methods AFP levels in the medium were measured for cultures of AFP-negative normal human cell line HL-7702 and AFP-positive human hepatoma cell lines BEL-7402,BEL-7404,Hep-G2,MHCC-97H and SMMC-7721.DNA was extracted and genotyped by real-time PCR（Taq-man assay）. AFP levels were compared for hepatoma cell lines with different genotypes. Results A statistically significant association was observed between rs 16849384 and AFP levels.AFP levels were higher in Hep-G2 cells carrying a T/T genotype at rs 16849384 than in SMMC- 7721,HL-7702 and BEL-7402 cells carrying a C/T genotype（P〈0.001 ,〈0.001 and〈0.015,respectively）. Conclusions Genotypes of rs 16849384 may be associated with AFP levels in hepatoma cell lines,and the T allele of rs 16849384 is associated with higher AFP level.

关 键 词：肝肿瘤 肝癌细胞株 甲胎蛋白 单核苷酸多态性 

分 类 号：R735.7[医药卫生—肿瘤]