复方枳椇子制剂对乙醇所致小鼠肝损伤的保护作用  

Protective effects of CPH on ethanol-induced liver injury in mice

作　　者：宋莎莎[1] 何伟[1] 袁平凡[1] 路景涛[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所抗炎免疫药物教育部重点实验室抗炎免疫药理学安徽省重点实验室抗炎免疫药物安徽省工程技术研究中心,安徽合肥230032

出　　处：《安徽医药》2013年第6期923-925,共3页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金项目(No 30973543;81173075);安徽高校省级自然科学产学研项目(No KJ2012A178);安徽高校省级自然科学重点项目(No KJ2012A160)

摘　　要：目的研究复方枳椇子制剂(CPH)对乙醇所致小鼠肝损伤的保护作用。方法采用56%乙醇灌胃的方法来诱导小鼠急性肝损伤模型。将小鼠随机分为空白组,模型组,CPH(1,2,4 g.kg-1)组和阳性(XJGGK)组。测定小鼠血清中谷丙转氨酶(ALT),谷草转氨酶(AST),甲胎蛋白(AFP),以及肝组织中丙二醛(MDA),超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)的含量或活性,并进行小鼠肝病理组织学检查。结果 CPH可显著降低饮酒小鼠血清中ALT、AST含量的升高,降低MDA含量,增加SOD活性和GSH-Px含量,并能改善乙醇损伤小鼠肝病理组织结构,但CPH提取物对饮酒小鼠AFP水平没有明显影响。结论 CPH对乙醇所致小鼠肝损伤具有保护作用,其机制可能与清除氧自由基和抗脂质过氧化有关,为将CPH开发成安全、有效的功能食品提供依据。Objective To study the protective effects of different ratio compatibility of CPH on acute ethanol-induced liver injury model in mice. Methods The acute liver injury animal model was established in mice by ethanol gavages（56% at 5 g ~ kg-1 ）. The levels of ALT, AST and AFP in serum and levels of MDA, SOD and GSH in liver tissue were measured and hepatic histological changes were ob- served by optical microscope in order to evaluate the protective effects of CPH. Results Compared with model group, the levels of ALT, AST and MDA content were significantly down-regulated, while SOD activity and GSH-Px content in liver tissue of acute alcoholism mice were up-regulated by CPH, but CPH couldn＇ t affect effectively the level of AFP. Histological results showed that CPH could ameliorate histological damages induced by ethanol in liver. Conclusion CPH has the protective effects on acute ethanol-induced liver injury in mice, and the mechanism may be associated with the elimination of free radicals and anti-lipid peroxidation, all of which may provide sig- nificant evidence for developing CPH into a safe and effective functional food.

关 键 词：复方枳椇子制剂 乙醇 肝损伤 抗脂质过氧化 

分 类 号：R285.5[医药卫生—中药学]