自体CIK细胞治疗中晚期消化道恶性肿瘤临床疗效评估  

Assessment of clinical effects of the treatment for middle and advanced stage gastrointestinal cancer with autologous CIK cells

作　　者：王志华[1,2] 陆宝石 焦义恒 王兴波 袁宏江 雷新生 王焕中 鲁祥石[2] 

机构地区：[1]合肥凤凰肿瘤医院生物免疫治疗中心,安徽合肥230001 [2]哈尔滨医科大学附属肿瘤医院,黑龙江哈尔滨150040

出　　处：《中国医药导报》2013年第21期86-89,共4页China Medical Herald

基　　金：国家自然科学基金项目(编号39270765);黑龙江省自然科学基金项目(编号D201171)

摘　　要：目的评估自体细胞因子诱导杀伤细胞(CIK)回输治疗中晚期消化道恶性肿瘤效果。方法选择2012年6月～2013年1月合肥凤凰肿瘤医院生物免疫治疗中心治疗的中晚期消化道恶性肿瘤患者118例,采用自体单个核细胞制备CIK细胞,培养2～3周后给予静脉输注治疗。通过荧光活化细胞分选法(FACS)测定患者治疗前后的免疫状态、生存质量(Karnofsky评分)、疾病控制率(DCR),观察毒副作用。结果经培养后,CIK细胞扩增较好,达到预期质量标准。患者治疗后CD4+、CD8+细胞比率较治疗前增加,差异有统计学意义(P<0.05);治疗后CD3+、CD4/CD8+,CIK细胞(CD3+/CD56+)高于治疗前,差异有高度统计学意义(P<0.01)。Kamofsky评分升高>20分者27例,10～<20分者29例,<10分者36例;下降<10分者26例。食管癌、胃癌、结肠癌DCR分别为55.26%(21/38)、64.91%(327/57)、65.22%(15/23)。无心、肝、肺、肾脏中毒反应。结论 CIK治疗恶性消化道肿瘤安全可靠,副作用小,疗效确切,并能有效改善患者细胞免疫状态,提高患者的生存质量,抑制肿瘤增长,提高治疗效果。Objective To assess the clinical effects for the treatment of middle and advanced stage gastrointestinal cancer with autologous CIK ceils. Methods 118 cases of middle and advanced stage gastrointestinal cancer from June 2012 to January 2013 in Biological Immune Therapy Center of Hefei Fenghuang Cancer Hospital were selected. Autologous mononuclear cells were used to prepare CIK ceils, intravenous infusion was carried out after culture for 2- 3 weeks. The immune state of patients before and after treatment were determined through FACS, quality of life for patients were scored according to Karnofsky, the index change of disease control rate （DCR） and side effects were carefully observed, the condition of toxic and side effect was observed. Results After culture, CIK cells were amplified well and the desired quality standards was achieved. CD4＋, CD8~ cell ratio after the treatment were higher than those before the treatment, the differences were statistically significant （P 〈 0.05）; CD3~, CD4/CD8~, CIK cells （CD3＋/CD56~） were higher than those before the treatment, the differences were statistically significant （P 〈 0.01）. 27 cases were found with the elevation of Kamofsky 〉 20 scores, 29 cases were found with the elevation of Kamofsky 10- 〈 20 scores, 36 cases were found with the elevation of Kamofsky 〈10 scores, 26 cases were found with the decline of Kamofsky 〈10 scores. The DCR of esophagus cancer, gastric cancer, colon cancer were 55.26% （21/38）, 64.91% （327/ 57）, 65.22% （15/23） respectively. Toxic reaction of heart, liver, lung, and kidney was not found. Conclusion CIK is safe, reliable, and it has small side effect and precise efficacy for treatment of malignant gastrointestinal tumors, also can effectively improve the cellular immune function and enhance the patient＇s quality of life and clinical efficiency.

关 键 词：细胞因子诱导杀伤细胞 生物治疗 消化道恶性肿瘤 

分 类 号：R730[医药卫生—肿瘤]