miR-223促进人胃癌MGC-803细胞增殖的研究  

Up-regulated miR-223 Promotes the Proliferation of Gastric Cancer MGC-803 Cells

作　　者：殷丹丹[1] 孔荣[1] 刘志军[1] 张二宝[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学与分子生物学系,江苏南京210029

出　　处：《现代生物医学进展》2013年第20期3814-3817,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81070620)

摘　　要：目的:探讨miR-223的表达与胃癌细胞的增殖能力的关系。方法:采用实时定量PCR检测人胃癌组织及细胞系中miR-223的表达。采用四甲基偶氮唑蓝(MTT)法和克隆形成实验测定转染miR-223 inhibitors前后的胃癌细胞增殖的变化。结果:相对于正常胃粘膜组织及细胞,在胃癌组织和细胞中miR-223的表达水平出现了显著的上调,MGC-803细胞中转染miR-223 inhibitors能显著抑制miR-223的表达水平。抑制miR-223能降低胃癌细胞的增殖能力。结论:miR-223对胃癌细胞增殖的调控至关重要。miR-223的表达降低能显著抑制胃癌细胞的增殖能力。Objective： To investigate the effects of miR-223 on gastric cancer cell proliferation. Methods： Real-time quantitative PCR was performed to detect the expression of miR-223 in human gastric cancer tissues and cell lines. Methyl thiazolyl tetrazolium （MTT） and colony formation assay were performed to detect the cell proliferation changes of the gastric cancer cells transfected with miR-223 inhibitors. Results： Compared with that in the normal gastric mucosa and cells, miR-223 up-regulated significantly in gastric cancer tissues and cells, miR-223 inhibitors transfection could significantly decrease its expression in MGC-803 cells. Inhibition of miR-223 can reduce the cell proliferation ability of gastric cancer cells. Conclusion： miR-223 is essential for gastric cancer cell proliferation. Reduced expression of miR-223 could significantly inhibit the proliferation of cancer cells.

关 键 词：胃癌 miR-223 细胞增殖 MTT 克隆形成 

分 类 号：R735.2[医药卫生—肿瘤]