Survivin-2B增强甲氨蝶呤抑制BEL-7402  

Survivin-2B Enhanced the Effects of Methotrexate on BEL-7402

作　　者：沈娟[1,2] 丁静[2] 黄演婷[2] 招巧莲[1] 杨政[2] 

机构地区：[1]广东药学院生命科学与生物制药学院,广东广州510006 [2]广东药学院基础学院,广东广州510006

出　　处：《现代生物医学进展》2013年第21期4035-4038,共4页Progress in Modern Biomedicine

摘　　要：目的:研究高表达Survivin-2B对化疗药物甲氨蝶呤抑制肝肿瘤细胞株BEL-7402作用的影响。方法:构建真核表达重组质粒Survivin-2B/pcDNA3.1(-),利用增强型绿色荧光蛋白pEGFP-C1真核质粒测定脂质体转染条件及效率,采用该条件瞬时转染Survivin-2B/pcDNA3.1(-)、Survivin/pcDNA3.1(-)以及pcDNA3.1(-)阴性对照质粒至肝肿瘤细胞株Bel-7402,MTT法检测100mg/ml甲氨蝶呤作用时,各组细胞抑制情况的差异。结果:成功构建Survivin-2B/pcDNA3.1(-)重组质粒,脂质体转染48 h转染效率为62%,甲氨蝶呤对高表达Survivin-2B的Bel-7402细胞抑制率最高。结论:Survivin-2B与甲氨蝶呤同时作用可以提高对Bel-7402细胞的抑制率,为肿瘤治疗提供了新思路。Objective： To investigate the effect of survivin-2B on the process of methotrexate killing BEL-7402tumor cell apoptosis.Methods： The eukaryotic expression recombinant plasmid pcDNA3.1（-）/ Survivin-2B was constructed.The enhanced cyan fluorescent protein was transfected to Bel-7402 by liposome to determine the transfection condition and efficiency.Then Survivin-2B/ pcDNA 3.1（-）,Survivin/ pcDNA3.1（-） was transfected to Bel-7402 by the same way.At last,the mortality of three groups was tested respectively by MTT method.Results： The pcDNA3.1（-）/ Survivin-2B vector was successfully constructed.Transfection efficiency was about 62 % at 48 h after transfected.The mortality of Bel-7402 group which highly expressed Survivin-2B was highest.Conclusion： Survivin-2B can increase the cytotoxicity of methotrexate against Bel-7402.It also provides a new way for tumor therapy.

关 键 词：剪接体 Survivin-2B SURVIVIN BEL-7402 

分 类 号：R730.54[医药卫生—肿瘤]