人参皂苷Rg3联合苏拉明通过下调TGF-β1表达抑制小鼠肺癌生长转移  

Inhibitory Effect of Ginsenoside Rg3 in Combination with Suramin on the Growth and the Metastasis of the Lung Cancer in Mice Via Down-regulation of TGF-β1

作　　者：张平[1] 许霞辉[1] 贺兼斌[2] 董素素[1] 阳洁[1] 刘理静[1] 

机构地区：[1]南华大学附属第一医院呼吸内科,湖南衡阳421001 [2]怀化市第一人民医院呼吸内科,湖南怀化418000

出　　处：《现代生物医学进展》2013年第21期4039-4043,共5页Progress in Modern Biomedicine

基　　金：2010年度湖南省卫生厅科研计划(B2010-133)

摘　　要：目的:肺癌是严重危害人类健康的恶性肿瘤之一,中晚期肺癌以放化疗为主,毒副作用大,观察低毒副作用的人参皂苷Rg3和苏拉明联合对小鼠Lewis肺癌生长转移的抑制作用及机制,为临床安全高效治疗肺癌提供实验资料。方法:将40只接种好Lewis肺癌细胞的C57BL6小鼠随机分为5组,每组8只:A组:对照组、B组:顺铂组、C组:人参皂苷Rg3组、D组:苏拉明组、E组:人参皂苷Rg3联合苏拉明组。接种第4天后开始药物干预,第20天处死,取肺组织,计肺转移结节数,剥离移植瘤,计瘤重,计算抑瘤率,通过RT-PCR及Western-blot检测移植瘤TGF-β1的mRNA及蛋白的表达。结果:各药物干预组的肺转移结节数及瘤重明显低于对照组(P<0.05,P<0.01),人参皂苷Rg3联合苏拉明组最明显;BCDE各组抑瘤率分别为:(19.69±5.61)%、(35.37±8.97)%、(35.85±7.55)%和(49.39±6.99)%,ABCDE各组的肺转移率分别为100%、100%、100%、87.5%和62.5%;各药物干预组的TGF-β1的mRNA及蛋白的表达均低于对照组(P<0.01),且人参皂苷Rg3联合苏拉明组TGF-β1的表达明显低于人参皂苷Rg3组和苏拉明组(P<0.05)。结论:人参皂苷Rg3联合苏拉明具有抑制小鼠Lewis肺癌的生长转移作用,对晚期肺癌的抑制可能与下调TGF-β1的表达有关。Objective： Lung cancer is a malignant tumor serious endangering human health.Radiotherapy and chemotherapy is the main treatment in metaphase or terminal lung cancer,but it has great toxic side effects.The aims of this study is to observe the inhibitory efficacy of Ginsenoside Rg3 in combination with Suramin on the growth and the metastasis of the Lewis lung cancer（LLC） in mice and the potential mechanisms,and supply the experiment data for the lung cancer clinical treatment with efficacy and safety.Methods： Highly metastasis Lewis lung cancer cells were inoculated into 40 C57BL6 mice to establish lung cancer models.The fourth day after inoculation,the mice were randomized into 5 groups（group A,B,C,D and E）,and received normal saline alone,cisplatin,ginsenoside Rg3,suramin,ginsenoside Rg3 combined with suramin therapy respectively for 16 days.After treatment completed,the metastasis nodules on lung surface and the transplantation tumor weight were measured in every group,and transforming growth factor-β1（TGF-β1） was detected by RT-PCR and western blot.Results： In cisplatin,suramin,ginsenoside Rg3,ginsenoside Rg3 combined with suramin groups,the transplantation tumor weight was suppressed obviously,with the tumor inhibitory rate of（19.69±5.61）%,（35.37± 8.97）%,（35.85±7.55）% and（49.39±6.99）%,respectively（P0.01）.The lung metastasis nodules were also decreased significantly in these therapy groups,especially in the combination group（P0.05）.The metastasis rate on lung surface was 100%,100%,100%,87.5% and 62.5% in group A,B,C,D and E respectively.The expression levels of TGF-β1 mRNA and protein in the transplantation tumor both was down-regulated in cisplatin,suramin,ginsenoside Rg3,ginsenoside Rg3 combined with suramin groups than in control group,and was also obviously in combination group（P0.05）.Conclusion： Ginsenoside Rg3 in combination with Suramin can inhibit effectively the growth and metastasis of LLC mice,which is involved in the suppression of TGF-β1 expr

关 键 词：LEWIS肺癌 人参皂苷RG3 苏拉明 TGF-Β1 

分 类 号：R734.2[医药卫生—肿瘤]