老年与青年大鼠大脑皮质及血清总胆固醇、三酰甘油和游离脂肪酸的对比分析  

Comparative analysis of total cholesterol,triglycerides and free fatty acids in cerebral cortex and serum between the old and young rats

作　　者：石如玲[1] 许娜[1] 杨彩玲[2] 王凡平[3] 范秉琳[1] 张煜[1] 翟伟科[4] 樊振琦[4] 冯玲玲[4] 

机构地区：[1]新乡医学院基础医学院,河南新乡453003 [2]新乡医学院第一附属医院口腔颌面外科,河南卫辉453100 [3]新乡医学院医学检验系,河南新乡453003 [4]新乡医学院,2010级本科河南新乡453003

出　　处：《新乡医学院学报》2013年第7期515-517,共3页Journal of Xinxiang Medical University

基　　金：河南省高等学校青年骨干教师资助计划项目(编号:2010GGJS-121);新乡医学院重点研究领域招标课题(编号:ZD2011-1);新乡医学院大学生科研创新计划项目(编号:DXSKYKT2011-010)

摘　　要：目的对比分析老年和青年大鼠大脑皮质及血清总胆固醇、三酰甘油和游离脂肪酸水平的变化。方法青年组(3月龄)和老年组(22月龄)大鼠各11只,大脑皮质及血清总胆固醇采用胆固醇氧化酶法测定,三酰甘油采用磷酸甘油氧化酶法测定,游离脂肪酸采用铜试剂显色法测定。结果与青年组大鼠比较,老年组大鼠大脑皮质和血清的总胆固醇水平显著增高(P<0.05),大脑皮质的三酰甘油水平显著增高(P<0.01),而大脑皮质和血清的游离脂肪酸水平显著降低(P<0.01)。结论大脑脂质成分的改变可能是脑老化的原因之一。Objective To analyze comparatively the changes of total cholesterol, triglyceride and free fatty acid levels in cerebral cortex and serum between the old and young rats. Methods Young （ 3 months old, n = 11 ） and old （22 months old, n = 11 ） male rats were used in this experiment. The level of total cholesterol in cerebral cortex and serum was detected by cho- lesterol oxidase method,the level of triglycerides was detected by phosphoglycerol oxidase method and the level of free fatty acid was detected by copper reagent chromogenic method. Results Compared with the young group, the level of total choles- terol in cerebral cortex and serum increased significantly（P 〈 0.05）, and the level of triglycerides in cerebral cortex increased significantly（ P 〈0.01 ） ,while the level of free fatty acid level in cerebral cortex and serum decreased significantly in the old group （P 〈 0. 01 ）. Conclusion Changes of lipid composition in old brain may be associated with cognitive decline during ag- ing.

关 键 词：衰老 大脑皮质 总胆固醇 三酰甘油 游离脂肪酸 

分 类 号：Q547[生物学—生物化学] R741[医药卫生—神经病学与精神病学]