大鼠骨内注射纳米羟基磷灰石颗粒在体内的分布特性  

Distribution Characteristics of Hydroxylapatite Nanoparticles via Intraosseous Administration in Rats

作　　者：汤京龙[1] 王硕[1] 刘丽[1] 王健[1] 奚廷斐[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中国药事》2013年第5期498-503,共6页Chinese Pharmaceutical Affairs

基　　金：国家自然科学基金(编号30770579);"973"项目(编号2007C13936101)

摘　　要：目的研究了大鼠骨内注射纳米羟基磷灰石(nano-HAP)颗粒后在体内的分布、蓄积和排泄情况,以了解nano-HAP是否具有在体内迁移的特性。方法试验分为nano-HAP和micro-HAP两组,分别将中子活化的nano-HAP和作为对照的微米羟基磷灰石(micro-HAP)注射到大鼠股骨骨髓腔中,剂量为18mg.kg-1。在2周、4周、12周、24周时处死动物,解剖并收集动物的血液、脑、心、肝、脾、肺、肾、胃、小肠、胸骨、睾丸、粪便和尿液。用高氯酸和双氧水将收集的样品消解后,通过液体闪烁计数器测定各脏器组织和排泄物中的45 Ca#射线计数来反映nano-HAP和micro-HAP在动物体内的分布。结果骨内注射nano-HAP后,大部分组织中45Ca#射线计数范围为0.0～32cpm.mg-1,注射micro-HAP后为0.0～27cpm.mg-1。两种HAP颗粒在体内分布部位方面没有显著差异,说明nano-HAP颗粒不会以颗粒形态在体内迁移,不具有在体内迁移的生物特性。骨骼是nano-HAP和micro-HAP唯一的蓄积器官,注射4周后,两组动物骨骼中的45Ca#射线计数变化不大。结论nano-HAP颗粒不具有在体内迁移的生物特性,可以在临床上得到更广泛的应用。Objective To investigate the distribution, accumulation and excretion of hydroxylapatite nanopartieles （nano-HAP） in rats with intraosseous administration so as to determine whether nano-HAP has the characteristic of in vivo migration. Methods Rats were divided into nano-HAP group and micro- HAP group. The neutron activated nano-HAP and hydroxylapatite microparticles （micro-HAP） as control were respectively injected into the rat femoral medullary cavity at a dose of 18 mg ~ kg-1 , and then the rats were sacrificed at 2 wk, 4 wk, 12 wk, 24 wk with the marrow broken off, and were dissected to collect their blood, brain, heart, liver, spleen, lung, kidney, small intestines, sternum, testicle, feces and urine. The collected samples were digested via perchloric acid and oxyful treatment, and then the counts of 45 Ca in every sample were measured by a liquid scintillation counter to obtain the distribution pattern of nano-HAP and micro-HAP within the animal bodies. Results Results indicated that 45 Ca detected in most organs was 0.0-32 cpm · mg^-1 after nano-HAP injection, and 0.0-27 cpm ·mg^-1 after micro- HAP injection. There were no significant differences between the SNP group and SMP group （P 〉0.05） in vivo distribution, indicating that nano-HAP does not have the biological characteristic of in vivo migrating in the form of particles. Bone is the only organ in which nano-HAP and micro-HAP accumulate, and the changes in ]3-ray counts of 4SCa in the bones of both groups were not significant. Conclusion The results suggest that nano HAP does not have the biological characteristic of in vivo migration, so it could be used more widely in biomedical applications.

关 键 词：纳米羟基磷灰石 体内分布 中子活化 大鼠 

分 类 号：O611.7[理学—无机化学] O615.4[理学—化学]