ALOX5AP基因多态性及肥胖与缺血性脑卒中相关性  

ASSOCIATION OF POLYMORPHISM OF SG13S32 IN ALOP5AP GENE AND OBESITY WITH ISCHEMIC STROKE

作　　者：李海先[1] 吴海容[1] 李健业[1] 周畅[1] 张晨[1] 

机构地区：[1]青岛大学医学院附属医院神经内科,山东青岛266003

出　　处：《青岛大学医学院学报》2013年第4期319-321,325,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省自然科学基金资助项目(ZR2010HM061)

摘　　要：目的探讨5-脂氧合酶激活蛋白(ALOX5AP)基因SG13S32C/A多态性及其与肥胖交互作用和缺血性脑卒中(IS)的相关性。方法采用病例对照研究的方法,以357例IS病人(IS组)和300例与其年龄、性别相匹配的健康体检者(对照组)为研究对象,应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)方法,检测SG13S32C/A位点基因多态性,比较两组基因型及等位基因的差异。用多因素Logistic回归去除混杂因素的影响,分析基因型、等位基因与IS的相关性,并分析其与肥胖的交互作用。结果 IS组与对照组SG13S32AA基因型、A等位基因比较差异有显著性(χ2=6.887、4.753,P<0.01);AA基因型合并肥胖增加IS的发生率(χ2=16.868,P<0.01)。结论 ALOX5AP基因中SG13S32C/A多态性与IS相关,A等位基因增加IS的发病风险。SG13S32变异基因型AA与肥胖在IS的发生中可能存在协同作用。Objective To explore the association of polymorphisms of SG13S32 （9551963） in the ALOXSAP and obesity with ischemic stroke （IS）. Methods A case-control study was applied in 357 IS patients and 300 non-IS controls who matched the patients＇ age and sex. The polymorphisms of SG13S32C/A were analyzed by using polymerase chain reaction and restrictive fragment length polymorphism （PCR-RFLP）, the difference of genotype and allele between the two groups was compared. The correlation between genotype, allele, and IS was investigated, and their association with obesity was analyzed by logistic regression to get rid of confounding variables. Results There were significant differences of SG13S32AA genotype and A allele between IS and the controls （x2=6.887,4,753;P〈0.01）. AA genotype complicating obesity increased the incidence of IS （x2=16.868,P〈0.001）. Conclusion The polymorphism of SG13S32C/A in ALOXSAP gene is correlated with IS, and A allele increases the risk of IS. A synergetic effect may exist between SG13S32 variant genotypes AA and obesity in the development of IS.

关 键 词：5-脂氧合酶激活蛋白 脑血管意外 多态性 单核苷酸 肥胖症 

分 类 号：R743.3[医药卫生—神经病学与精神病学]