胺碘酮对兔心肌缺血再灌注后血清SOD和MDA影响  

EFFECTS OF AMIODARONE ON SERUM SOD AND MDA AFTER MYOCARDIAL ISCHEMIA-REPERFUSION IN RABBITS

作　　者：信栓力[1,2] 杨华[2] 常超[1] 王晓丽[1] 刘丽军[1] 孟利民[1] 赵秀峰[1] 段丽萍[1] 

机构地区：[1]邯郸市第一医院心内科,河北邯郸056002 [2]河北医科大学研究生学院

出　　处：《齐鲁医学杂志》2013年第3期230-231,234,共3页Medical Journal of Qilu

基　　金：河北省科技计划项目(09276101D-8)

摘　　要：目的探讨胺碘酮对兔心肌缺血再灌注后血清超氧化物歧化酶(SOD)和丙二醛(MDA)水平的影响及意义。方法将新西兰大白兔随机分为假手术组、对照组、胺碘酮组,假手术组开胸只穿线不结扎血管,后两组开胸并结扎兔左冠状动脉回旋支制备缺血再灌注无复流模型,每组10只。对照组再灌注即刻给予等量的生理盐水,胺碘酮组再灌注即刻给予胺碘酮3mg/kg。分别于再灌注后120min取血,测定各组血清SOD活性和MDA含量。结果在缺血范围差异无显著性的条件下,胺碘酮组无复流范围显著小于对照组(t=4.483,P<0.05)。与假手术组相比,对照组及胺碘酮组再灌注后血清中SOD活性明显降低(F=800.90,q=18.89、55.65,P<0.01),MDA含量明显增加(F=1 457.60,q=55.13、73.32,P<0.01);与对照组相比,再灌注后胺碘酮组血清中SOD活性明显增高(q=36.76,P<0.01),MDA含量明显降低(q=18.18,P<0.01)。结论胺碘酮可能是通过抑制氧自由基的产生,改善心肌缺血再灌注损伤,减轻无复流损伤的程度。Objective To assess the influence of Amiodarone on serum superoxide dismutase （SOD） and malondialde hyde （MDA） in rabbits and its significance after myocardial ischemia/reperfusion （I/R）. Methods Thirty mature rabbits were evenly randomized to sham-operation group, control group and amiodarone group. Open chest surgery onl^without legating the blood vessels-was done for rabbits in the sham-operation group. For those in the latter two groups, left circumflex arteries were li- gated to create an I/R model. The model in the control was given reperfusion of equivalent normal saline, and those in amiodarone group were offered amiodarone 3 mg/kg. Serum SOD and MDA were measured after 120min of reperfusion. Results Under si- milar condition of ischemic range, the no-reflow area in amiodarone group was much smaller than that in the control （t = 4.483, P〈0.05）. Compared with shame operation group, the activity of serum SOD in the control and amiodarone groups after re-perfu- sion was significantly lowered （F：800.90;q=18.89,55.65;P〈0.01）, and MDA elevated （F：I 457.60;q--55.13,73.32;P〈0.01） ; Compared with the control, the activity of SOD in amiodarone group was significantly elevated （q- 36.76, P 〈0.01 ）, and content of MDA lowered （q=18.18, P 〈 0.01）. Conclusion Amiodarone relieves the extent of no-reflow damage is probably through inhibiting the generation of oxygen-derived free radicals and ameliorating myocardial I/R injury.

关 键 词：胺碘酮 再灌注损伤 超氧化物歧化酶 丙二醛 

分 类 号：R743.3[医药卫生—神经病学与精神病学]