ALOX5AP、COX2和MMP9基因多态性与缺血性脑卒中相关性  

ASSOCIATION BETWEEN GENE POLYMORPHISM OF ALOX5AP,COX2,MMP9 AND ISCHEMIC STROKE

作　　者：李健业[1] 吴海容[1] 李海先[1] 侯树刚[1] 孙瑛[1] 周畅[1] 张晨[1] 

机构地区：[1]青岛大学医学院附属医院神经内科,山东青岛266003

出　　处：《齐鲁医学杂志》2013年第4期321-324,共4页Medical Journal of Qilu

基　　金：山东省自然科学基金资助项目(ZR2010HM061)

摘　　要：目的探讨5-脂氧合酶激活蛋白(ALOX5AP)、环氧化酶2(COX2)和基质金属蛋白酶9(MMP9)基因单个位点、单倍型及基因-基因间的相互作用与缺血性脑卒中(IS)的相关性。方法检测302例IS病人及308例健康查体者(对照组)ALOX5AP89G/A、COX2-765G/C、COX2-1195G/A以及MMP9-1562C/T的基因多态性,并对各基因位点及COX2基因两个位点的单倍型进行分析。用广义多因子降维(GMDR)方法检测基因与基因之间的相互作用。结果COX2-765GC基因型和COX2-1195GA基因型均与IS的患病风险相关(校正后OR=2.650、1.565),COX2-765C等位基因和COX2-1195G等位基因增加IS患病风险(OR=2.142、1.344);IS组单倍型C-765A-1195和G-765G-1195较对照组高(OR=1.872、1.289),而单倍型G-765A-1195较对照组低(OR=0.649);IS组和对照组ALOX5AP89G/A和MMP9-1562C/T位点等位基因、基因型的频率差异均无显著性(P>0.05)。GMDR检测显示,COX2基因和MMP9基因间相互作用有统计学意义,但多元Logistic回归没有进一步证实这种相互作用。结论汉族人群IS可能与COX2基因多态性有关,ALOX5AP基因和MMP9基因多态性与IS可能无关;IS病人COX2基因和MMP9基因间可能没有相互作用。Objective To explore whether genetic variants in the ALOX5AP gene, COX2 gene and MMP9 gene would synergistically influence the risk of ischernie stroke （IS）. Methods Variants of ALOX5AP89G/A, COX2-765G/C, COX2- l195G/A and MMPg-1562C/T in 302 IS patients and 308 healthy controls were detected, and the polymorphisms studied. By using GMDR, the interaction among genes was measured. Results COX2-765GC genotype （adjusted OR =2.650） and COX2-1195GA genotype （adjusted OR = 1.565） were associated with the risk of IS. In IS group, C-765A1195 and G-765G-1195 were higher than that in the control （0R=1.872,1.289）, while G-965A-1195 was lower （OR = 0.649）; The differences of ALOX5AP89G/A and MMP9- 1562C/T allele, and genotype frequency between IS and control group were not significant （P〉0.05）. The GMDR analysis showed a statistically significant gene interaction between COX2 and MMP9, but which was not confirmed by Logistic regression analysis. Conclusion In Han people, IS may associate with genetic polymorphism of COX2, and not with ALOXSAP and MMPg. There is probably no interaction of COX2 and MMP9 in IS.

关 键 词：5-脂氧合酶激活蛋白 环氧化酶2 明胶酶B 脑血管意外 遗传 多态性 单核苷酸 

分 类 号：R743[医药卫生—神经病学与精神病学]