白三烯C4合成酶基因多态性与缺血性脑卒中关系  

ASSOCIATION BETWEEN LEUKOTRIENE C4 GENE POLYMORPHISMS AND CEREBRAL ISCHEMIC STROKE

作　　者：孙瑛[1] 李海先[1] 吴海容[1] 侯树刚[1] 周畅[1] 张晨[1] 

机构地区：[1]青岛大学医学院附属医院神经内科,山东青岛266003

出　　处：《齐鲁医学杂志》2013年第4期328-330,共3页Medical Journal of Qilu

基　　金：山东省自然科学基金项目(ZR2010HM061)

摘　　要：目的探讨白三烯C4(LTC4)合成酶基因启动子区rs730012(-444A/C)位点多态性与缺血性脑卒中(IS)的相关性。方法应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)及凝胶电泳技术,检测IS组和与其性别及年龄相匹配的健康对照组LTC4合成酶基因启动子区rs730012位点多态性,并比较两组基因型、等位基因频率的差异。采用Logistic回归去除传统危险因素的影响,分析其基因型、等位基因频率与IS发病相关性。结果IS组rs730012位点非携带C等位基因(AA基因型)人群患IS的风险约是携带C等位基因(AC+CC基因型)人群的1.6倍(OR=1.636,P<0.05)。结论 LTC4合成酶基因rs730012位点多态性可能与IS发病相关,等位基因A可能是其风险等位基因。Objective To elucidate the relationship between leukotriene C4 synthetase rs730012 （-444A/C） gene poly- morphism and cerebral ischemic stroke （CIS）. Methods With polyrnerase chain reaction restriction fragment length polymor phism （PCR-RFLP） and agarose gel electrophoresis techniques, the leukotriene C4 synthetase rs730012 gene polymorphism in CIS group and in age and sex-matched control group were detected and compared in terms of the differences of genotype and allele frequency between the two groups. The association of the gene polymorpblsm and the occurrence of CIS was analyzed by logistic re gression to adjust confounding variables. Results Logistic regression analysis showed that population with AA genotype of rs730012 had a 1.6 fold higher risk of suffering from CIS than tbose with AC＋CC genotypes （C）R = 1.636, P〈0.05）. Conclusion The polymorphisms of rs730012 in leukotriene C4 synthetase may be related to CIS. Allele A is probably the risk allele.

关 键 词：白三烯C4 脑血管意外 多态性 单核苷酸 

分 类 号：R743[医药卫生—神经病学与精神病学]