葛根素抑制痴呆大鼠模型海马神经元凋亡的研究  

PUERARIN INHIBITION ON HIPPOCAMPAL NEURONAL APOPTOSIS IN DEMENTIA RAT MODEL

作　　者：王文胜[1] 王英杰[2] 宋纯彦[1] 王连芹[1] 王建民[1] 胡沛霖[1] 

机构地区：[1]河北省邢台市人民医院神经内一科,河北邢台054000 [2]河北省邯郸市第一医院神经内三科,河北邯郸056000

出　　处：《河北医科大学学报》2013年第7期753-756,F0003,共5页Journal of Hebei Medical University

摘　　要：目的用痴呆大鼠模型研究海马神经元凋亡机制及葛根素对其干预效应。方法雄性Wistar大鼠36只被随机分为对照组、痴呆组、治疗组,用HE染色、TUNEL染色和流式细胞技术观察痴呆大鼠模型海马神经元的凋亡现象及葛根素的影响,采用免疫组织化学染色和Western.Blot分析,研究其分子机制是否涉及凋亡基因Bax、Bcl-2和凋亡蛋白酶caspase-3表达变化。结果①)HE染色、TUNEL染色时,对照组海马凋亡神经元少见;流式细胞术测定大鼠海马凋亡细胞率显示,对照组大鼠海马的凋亡细胞率处于较低水平,痴呆组与对照组比较海马凋亡神经元数量明显增加,凋亡细胞率明显增加(P<0.05),治疗组与痴呆组比较海马凋亡神经元数量明显减少,凋亡细胞率明显降低(P<0.05)。②痴呆组比对照组大鼠海马组织Bax、caspase-3蛋白的表达量明显增加(P<0.05),Bcl-2蛋白表达量明显减少(P<0.05);治疗组与痴呆组相比大鼠海马组织Bax、caspase-3的表达量明显降低(P<0.05),Bcl-2蛋白表达量明显增加(P<0.05)。结论葛根素能抑制痴呆大鼠模型海马神经元凋亡,可为葛根素治疗痴呆提供理论依据。Objective To investigate hippocampal neuronal apoptosis mechanisms and effect of puerarin＇s intervention in dementia rat model. Methods Thirty-six male Wistar rats we＇re randomly divided into control group, dementia group, treatment group. HE staining, TUNEL staining and flow cytometry were used to observe the hippocampal neuronal apoptosis and effect of puerarin in dementia rat model ,immunohistochemical staining and Western Blot analysis were performed to study the molecular mechanisms involved in apoptosis genes Bax, Bcl-2 and apoptotic protease caspase-3 expression.Results （1） In HE staining and TUNEL staining, apoptotic neurons in the hippocampus were rare in control group. Apoptotic cell rate of the hippocampus was measured by flow cytometry. The apoptotic rate in control group was at a low level. The number and the rate of apoptotic neurons were significantly increased in dementia group compared with those in control group （ P 〈 0.05 ）, while the number and the rate of apoptotic neurons in treatment group decreased significantly compared with those in dementia group （ P 〈 0.05 ）. （2）Hippocampal tissue Bax and caspase-3 protein expression were significantly increased （P 〈 0. 05）, Bcl-2 protein expression was significantly reduced （ P 〈 0.05 ） in dementia group compared with control group. In treatment group, hippocampal tissue Bax and caspase-3 protein expression were significantly reduced （P 〈 0.05 ）, Bcl-2 protein expression was significantly increased （P 〈 O. 05 ） compared with those in dementia group. Conclusion Puerarin can inhibit hippocampal neuronal apoptosis in dementia rat model and it provide a theoretical basis for puerarin in the treatment of dementia.

关 键 词：阿尔茨海默病 葛根素 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]