亚硒酸钠保护游离脂肪酸诱导肝细胞功能和Caspase3活性的变化研究  

The protective effects of sodium selenite on liver function and the activity of Caspase 3 induced by free fatty acids

作　　者：张鹏春[1] 陈丽君[1] 范瑞威[1] 郭苗苗[1] 郭志侯[1] 张志刚[1] 

机构地区：[1]东北农业大学动物医学学院

出　　处：《中国饲料》2013年第14期15-18,共4页China Feed

基　　金：国家自然科学基金青年项目(31101868);黑龙江省青年科学基金(QC2010057);中国博士后科学基金特别资助(2012T50302);东北农业大学大学生科创基金(2011056)

摘　　要：为研究亚硒酸钠(Na2SeO3)对游离脂肪酸(FFA)诱导大鼠肝细胞功能和Caspase 3活性变化的作保护用,采用胶原酶两步灌流法分离培养肝细胞,在细胞培养液中添加FFA和Na2SeO3(0.10μmol/L),测定肝细胞上清液生化指标、细胞增殖率和Caspase 3活性。结果表明,培养液中添加FFA,细胞增殖率降低,肝细胞功能受到影响,Caspase 3活性升高,且FFA的这种作用呈剂量依赖性;Na2SeO3能抑制由FFA引起的肝细胞的上述变化。The experiment was conducted to study the effect of sodium selenite on liver function and the activity of Caspase 3 induced by free fatty acids （FFA）.Primary rat hepatocytes were obtained by two-step collagenase perfusion method.Cell proliferation rates,liver function and the activity of Caspase 3 in FFA-treated group with or without sodium selenite （0.10 μmol/L） were determined.The results showed that：FFA changed doseependently cell proliferation rates, liver function and the activity of Caspase 3, whereas the presence of sodium selenite reversed these alternations.

关 键 词：亚硒酸钠 游离脂肪酸 细胞增殖率 肝细胞功能 CASPASE 3 

分 类 号：S816.7[农业科学—饲料科学]