超滤-毛细管电泳测定肺癌患者血清中的唾液酸  

Determination of Sialic Acids in Serum of Lung Cancer with Ultrafiltration-Capillary Electrophoresis

作　　者：董秀玲[1,2] 戴忠鹏[1] 林炳承[1] 鞠秀君[3] 王晓伟 袁小林[3] 

机构地区：[1]中国科学院大连化学物理研究所 [2]大连市第三人民医院,辽宁大连116033 [3]大连市第三人民医院

出　　处：《色谱》2000年第5期426-428,共3页Chinese Journal of Chromatography

基　　金：国家自然科学基金!资助项目 (2 96350 2 0 )

摘　　要：建立了用超滤 毛细管电泳技术测定血清中唾液酸含量的方法 ,并考察其最佳操作条件。该方法分离分析唾液酸可直接用紫外检测 (1 95nm) ,不需要柱前、柱后衍生。方法的回收率为 92 6%,最低检测浓度为 9 6μmol/L ,最低检测量为 39fmol。用该法测定了 30例正常人和 1 1例肺癌患者血清中唾液酸的含量 ,结果表明后者比前者有显著性增加 (P <0 0 0 1 )。用该方法测定的结果与传统使用的比色法结果之间呈良好的线性关系 (r=0 983,n =1 0 )。方法简单、灵敏 ,适合恶性肿瘤防治的基础研究和临床应用。A method for the determination of sialic acids in serum with ultrafiltration capillary electrophoresis is described and the operation was optimized Sialic acids were directly separated and analyzed with UV detection at 195 nm and without pre or post column derivatization The recovery of N acetylneuraminic acid (NANA) was 92 6%, the concentration and mass detection limit of NANA were 9 6 μmol/L and 39 fmol respectively This method was used for the determination of NANA level in the serum of 11 lung cancer patients and 30 normal adults The results showed that the average concentration of NANA in the serum of patients was much higher than that of normal adults with P <0 001 The results were also compared with those obtained by the traditional colorimetric method, with good linear relationship of r=0 983 at n =10 It is concluded that the method described in this paper is simple and sensitive, and is suitable for basic research and clinical applications to malignant tumors

关 键 词：毛细管电泳 超滤作用 唾液酸 肺癌 血清 测定 

分 类 号：R730.43[医药卫生—肿瘤] R446.1[医药卫生—临床医学]