高灵敏荧光增敏法测定抗坏血酸  

Highly Sensitive Fluorescent Enhance Assay for Ascorbic Acid

作　　者：王全林[1] 刘志宏[1] 毛陆原[1] 蔡汝秀[1] 

机构地区：[1]武汉大学化学与环境科学学院,武汉430072

出　　处：《分析化学》2000年第10期1229-1232,共4页Chinese Journal of Analytical Chemistry

摘　　要：基于痕量抗坏血酸对模拟酶β－ＣＤ－ｈｅｍｉｎ催化Ｈ２Ｏ２氧化对甲基酚体系的产物荧光的增强作用，提出了一种新的测定抗坏血酸荧光分析方法。方法具有很高的灵敏度和较好的选择性，其线性范围为１×１０－９～８×１０－８ｍｏｌ／Ｌ。检出限可达３．４５×１０－１０ｍｏｌ／Ｌ。用于实际样品的分析。A new spectrofluorimentric method with very high sensitivity and good selectivity for determining trace amount of ascorbic acid (AA) has been proposed. The method is based on the enhancement of AA on the fluorescence intensity of the product yielded in the redox reaction between p- cresol and bydrogen peroxide, in which β-CD-hemin acts as the mimetic enzyme of peroxidase. linear calibration graph was obtained over the AA concentration range 1 × 10-9 mol/L- 8 × 10-8 mol/L with a detection limit of 3.45 × 10-10 mol/L of AA. The method has ho applied in the analysis of real samples with satisfactory results.

关 键 词：抗坏血酸 荧光法 模拟酶催化 测定 HRP 

分 类 号：O629[理学—有机化学]