两种不同方法固定化β-葡萄糖苷酶的研究  

Immobilization of β-Glucosidase by chitosan

作　　者：虎亚芳[1] 丁克毅[1] 刘军[1] 

机构地区：[1]西南民族大学化学与环境保护工程学院,四川成都610041

出　　处：《西南民族大学学报（自然科学版）》2013年第4期520-524,共5页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省应用基础研究计划项目(2012JY0098);西南民族大学中央高校基本科研业务费专项资金项目(12ZYXS75)

摘　　要：以自制的壳聚糖微球为载体,戊二醛为交联剂,考察了壳聚糖浓度对微球制备的影响,并采用吸附-交联法和交联-吸附法两种方法对β-葡萄糖苷酶固定化进行了研究.结果表明:(1)当壳聚糖:2%乙酸溶液=1:20(w/w)时,最适合制球,成球粒径平均约为1.5mm.(2)采用交联-吸附法时固定率为43%,储存5天后剩余酶活力为67%,10天后剩余酶活力为52%,采用吸附-交联法时固定率为32%,储存5天后剩余酶活力为60%,10天后剩余酶活力为43%.因此,优化的固定化β-葡萄糖苷酶的方法为交联-吸附法.β-Glucosidase is immobilized by using chitosan microspheres as carrier, cross-linked with glutaraldehyde. The influence of chitosan concentration on the chitosan microsphere preparation -is investigated, the methods of enzyme immobilization, i.e., adsorption - crosslinking and crosslinking- adsorption for β- glycosidase is studied. The results indicate that the ratio of chitosan： 2% acetic acid solution =1：20（w/w） is most suitable ratio for the preparation of chitosan microsphere, the average diameter of the chitosan microsphere is 1.5mm, and that the fixed rate of crosslinking-absorption is 43%, the residual enzyme activity is 67% after 5 days, while the residual enzyme activity is 52 % after l0 days. For absorption-cross-linking method, the fixed rate is 32%, the residual enzyme activity is 60% after 5 days, while the residual enzyme activity is 43 % after 10 days. So, crosslinking-adsorption is chosen as the best method to immobilize β-glycosidase enzymes.

关 键 词：壳聚糖 Β-葡萄糖苷酶 固定化 

分 类 号：O643.36[理学—物理化学] Q814.2[理学—化学]