苏云金芽胞杆菌中aiiA基因密码子偏好性分析  

Analysis on Codon Usage Bias of aiiA Gene from Bacillus thuringiensis

作　　者：曾世涌[1] 苏小惠[1] 谢盼盼[1] 毛惠民[1] 杨梅[1] 

机构地区：[1]福建师范大学生命科学学院,福州350108

出　　处：《生物技术进展》2013年第4期257-263,共7页Current Biotechnology

基　　金：福建省自然科学基金项目(2012J01123);福建省教育厅项目(JK2012009);生物学拔尖人才班训练项目(201011)资助

摘　　要：aiiA基因指导表达的AiiA蛋白是一类能水解细菌群体感应信号分子内酯键的内酯酶。本研究使用CodonW、CUSP和CHIPS等相关程序对苏云金芽胞杆菌中aiiA基因的密码子使用情况进行分析,比较了aiiA基因与大肠杆菌、苏云金芽胞杆菌、枯草芽胞杆菌以及毕赤酵母的密码子偏好性。结果表明,aiiA基因偏好使用以A、T结尾的密码子;aiiA基因的密码子用法与大肠杆菌、苏云金芽胞杆菌、枯草芽胞杆菌和毕赤酵母的基因组密码子用法都有不同程度的偏好差异,其中与毕赤酵母的密码子用法差异最大;比较了aiiA基因分别以苏云金芽胞杆菌、大肠杆菌、枯草芽胞杆菌及毕赤酵母为宿主时密码子使用频率,发现都含有不同程度的低频密码子聚集现象,如果要在上述几种表达系统中实现aiiA基因的高效表达则需对aiiA基因的部分密码子进行优化改造。AliA, encoded by aiiA gene is one of the homoserine lactone enzyme that can hydrolyze the AHL on the lactone ring of Gram-negative bacteria. Coding sequence of aiiA gene from Bacillus thuringiensis was analyzed by CodonW, CUSP and CHIPS programs for identifying codon bias. Comparing the codon usage of aiiA gene with genomic codon usage of Escherichia coli, Bacillus thuringiensis, Bacillus subtilis and Pichia pastoris, it was indicated that aiiA gene prefers to use the codons ended with A or T; codon bias of aiiA gene from Bacillus thuringiensis was significantly different from those of E. coli, Bacillus subtilis and Pichia pastoris. The result of comparing the usage frequency of aliA gene in four different hosts showed that there were different degrees of low frenquency regions. The analytic result was very important guideline for designing the aiiA gene to improve the expression of AiiA in those four express systems.

关 键 词：苏云金芽胞杆菌 AIIA基因 密码子偏好性 密码子优化 异源表达 

分 类 号：Q933[生物学—微生物学]