结肠癌中Destrin表达及临床病理学意义  

Expression of Destrin in human colon cancer and its clinicopathologic significance

作　　者：苏坚[1,2] 颜鸿飞[2,3] 周钰娟[2] 廖前进[2] 史玲[2] 杨晶[2] 苏琦[2] 

机构地区：[1]南华大学附属第二医院病理科,衡阳421001 [2]南华大学肿瘤研究所/湖南省高校肿瘤细胞与分子病理学重点实验室,衡阳421001 [3]南华大学附属南华医院病理科,衡阳421001

出　　处：《临床与实验病理学杂志》2013年第7期713-716,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(31000629;81102854);湖南省卫生厅科研项目(B2008-067)

摘　　要：目的探讨Destrin蛋白表达与结肠癌发生、分化程度、肿瘤大小、淋巴结转移、临床分期的关系。方法将87例结肠癌、26例结肠上皮内瘤变及34例正常结肠组织标本制成组织芯片,采用免疫组化SP法检测Destrin表达。结果 Destrin在正常结肠黏膜中的阳性率为20.59%(7/34),低于结肠上皮内瘤变(42.31%),二者差异无显著性(P>0.05)。Destrin在低级别上皮内瘤变中表达低于高级别上皮内瘤变,二者差异无显著性(P>0.05)。Destrin在高级别上皮内瘤变组织中的表达明显高于正常结肠黏膜组织(P<0.05);Destrin在结肠癌中阳性率为80.46%(70/87),明显高于正常结肠黏膜和上皮内瘤变(P<0.05)。Destrin在高、中、低分化腺癌中的阳性率分别为70.00%(7/10)、80.85%(38/47)与83.33%(25/30),三者之间差异无显著性(P>0.05)。结肠癌中Destrin表达与肿瘤大小、淋巴结转移和TNM分期密切相关(P<0.05)。结论 Destrin表达与结肠癌的发生、肿瘤大小、淋巴结转移和临床分期密切相关,其可能是结肠癌诊断及预后的重要生物学标志物。Purpose To investigate the relation of Destrin expression with carcinogenesis, differentiation, tumor size, lymph node me- tastasis and clinical stage in human colon cancer. Methods Immunohistochemistry method was used to examine the expression of De- strin in colon cancer （87 cases）, intraepithelial neoplasia （IN） （26 cases） and normal tissue （34 cases） with tissue chip including 147 cases of colon specimens. Results The expression of Destrin in normal tissue was 20.59% （7/34） which was lower than that in IN （42. 31% ）, but no significant difference was observed （ P 〉 0. 05 ）. The expression of Destrin in low-grade IN was lower than that in high-grade IN, but there was no significant difference （ P 〉 0. 05 ）. Destrin expression in high-grade IN was higher than that in nor- mal tissue （P 〈 0. 05 ）. Destrin expression in colon cancer was 80. 46% （70/87）, which was significantly higher than that in normal tissue and IN, respectively （ P 〈 0. 05 ）. Destrin expression in well-differentiated, moderately differentiated and poorly differentiated adenocarcinoma was 70.00% （7/10）, 80. 85% （38/47） and 83.33% （25/30）, respectively, however, there was no significance difference （P 〉 0. 05 ）. Destrin expression in I ＋ II stage was significantly lower than that in Ⅲ ＋ Ⅳ（ P 〈 0. 05 ）. There was no sig- nificantly correlation between age and the expression of Destrin （ P 〉 0. 05 ）. Conclusions The expression of Destrin is closely related to carcinogenesis, tumor size, lymph node metastasis and clinical stage in colon cancer, which it suggest that Destrin may be a impor- tant biomarker of diagnosis and prognosis of colorectal carcinoma.

关 键 词：结肠肿瘤 Destrin 组织芯片 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]