肿瘤出芽对判断pT3期结直肠高、中分化腺癌淋巴结转移的价值  

Value of tumor budding in determining lymph node metastasis in pT3 well- and moderatelydifferentiated colorectal adnocarcinoma

作　　者：赵有财[1] 王劲松[1] 黄悦[1] 李俐[1] 杨小兵[1] 赵建华[1] 黄文斌[1] 

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)病理科,南京210006

出　　处：《临床与实验病理学杂志》2013年第7期730-732,736,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：南京医科大学科技发展基金(09NJMUM080);南京市卫生局医学科技发展项目(YKK11115)

摘　　要：目的探讨pT3期结直肠高、中分化腺癌肿瘤出芽数目及其与临床病理参数之间的关系。方法采用光镜和免疫组化EnVision法观察106例结直肠癌组织中肿瘤出芽情况并进行分级。结果肿瘤出芽数:1级37例(34.9%),2级27例(25.5%),3级42例(39.6%)。肿瘤出芽数1级组(<5个/HPF)的淋巴结转移检出率明显低于2级组(5～9个/HPF)和3级组(≥10个/HPF),差异有显著性(P=0.000)。3级组淋巴结转移检出率高于2级组,差异无统计学意义(P=0.206)。肿瘤出芽分级与患者年龄、性别及肿瘤发生部位、分化程度均无关(P>0.05)。结论 pT3期结直肠高、中分化腺癌中,肿瘤出芽数≥5个/HPF是提示肿瘤淋巴结转移的重要组织学特征。Purpose To study the relation between tumor budding and clinicopathologic parameters in pT3 well- and moderately-differ- entiated colorectal adnocarcinoma. Methods To detected the tumor budding of 106 cases of pT3 well- and moderately-differentiated colorectal adnocarcinoma by HE staining and EnVision immunohistochemical staining, and divided these into three grades by their in- tensity. Results Tumor budding of grade 1 was detected in 37 cases （34. 9% ）, tumor budding of grade 2 was detected in 27 cases （25.5%）, tumor budding of grade 3 was detected in 42 cases （39.6%）. The lymph node metastasis detection rate of tumor budding grade 1 group was significantly lower than that of tumor budding grade 2 and grade 3 groups, the difference was significant （P = 0. 000）. The lymph node metastasis detection rate of tumor budding grade 3 group was higher than that of tumor budding grade 2 group, but no significant difference （P = 0. 206）. Tumor budding grading was not associated with patients＇ age, sex, tumor location and differentiation （ P 〉 0. 05 ）. Conclusion The number of tumor budding I〉 5/HPF is an important histological characteristic to pre- dict lymph node metastasis in the pT3 well- and moderately- differentiated colorectal adenocarcinoma.

关 键 词：结直肠肿瘤 肿瘤出芽 病理学 免疫组织化学 

分 类 号：R737.25[医药卫生—肿瘤]