K-ras基因状态与结直肠癌临床病理特征的关系  

Relationship between K-ras mutational status and clinical pathological characteristics in colorectal cancer

作　　者：杨瑞钦[1] 杨春康[1,2] 徐可[1] 许元基[1] 

机构地区：[1]福建医科大学省立临床医学院,福州350014 [2]福建省肿瘤医院腹部外科,福州350014

出　　处：《临床与实验病理学杂志》2013年第7期741-744,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的观察结直肠癌原发灶K-ras基因的突变,探讨其与临床病理特征的关系。方法运用实时荧光定量PCR法检测230例结直肠癌组织K-ras基因12、13密码子的突变,利用χ2检验分析其与临床病理特征的相关性。结果 230例结直肠癌患者中,84例K-ras基因发生突变,突变率为36.5%,其中12密码子突变65例(28.2%)、13密码子突变19例(8.3%)。结直肠癌肺转移患者K-ras基因突变率较无肺转移患者高(P=0.022),12、13单密码子突变与临床病理特征(患者年龄、性别、肿瘤部位、病理分型、TNM分期、Dukes分期、区域淋巴结及肝肺转移)无关(P>0.05)。结论结直肠癌K-ras基因突变可能与肺转移存在相关性,检测K-ras基因突变对结直肠癌患者临床个体化治疗具有指导意义。Purpose To detect K-ras gene mutational status in colorectal primary tumor and to discuss the correlation between K-ras gene mutational status and clinical pathological features. Methods K-ras 12 and 13 codon mutational status in 230 cases of colorectal primary tumor were detected by real-time fluorescence quantitative PCR method, then the associations between K-ras mutation status and clinical pathological characteristics was analysed by Chi-square test. Result Of 230 cases of eolorectal carcinoma, 84 cases had K-ras mutation and the total mutation rate of K-ras was 36. 5%. 65 cases were K-ras 12 codon mutation （28.2%） and 19 cases were 13 codon mutation （8.3%）. The rate of K-ras gene mutation in cases with pulmonary metastases is significantly higher than those with- out pulmonary metastasis （P = 0. 022）. There were no relationship between the 12 or 13 codon mutation and clinical pathological char- acteristics （ P 〉 0.05 ）. Conclusion K-ras mutation may play a role in colorectal cancer pulmonary metastasis. The detection the K- ras mutation in colorectal carcinoma can provide information for clinical individualized treatment.

关 键 词：结直肠肿瘤 肺转移 K—ras 

分 类 号：R735.3[医药卫生—肿瘤]