穿心莲内酯对人前列腺癌DU145细胞的增殖抑制及凋亡诱导作用的体外研究  

Andrographolide Inhibits Proliferation and Induces Apoptosis in Human Prostate Cancer DU145 Cells in Vitro

作　　者：潘良朋[1] 曹余光[1] 

机构地区：[1]辽宁医学院附属第三医院泌尿外科,辽宁锦州121000

出　　处：《南昌大学学报（医学版）》2013年第4期5-8,14,F0003,共6页Journal of Nanchang University：Medical Sciences

摘　　要：目的探讨穿心莲内酯(Andro)在体外对人前列腺癌DU145细胞抗肿瘤的作用及机制。方法倒置显微镜下观察DU145细胞在Andro(浓度为5.0μmol.L-1)作用24h前后细胞形态的变化;MTT法检测Andro(浓度为5.0、10、20、40和80μmol.L-1)作用于体外培养24、48和72h后DU145细胞的增殖抑制率;AnnexinV-FITC/PI双染后流式细胞术检测Andro(浓度为10、20和40μmol.L-1)处理24h后DU145细胞的凋亡率;Westernblotting法分析Andro(浓度为10、20、40μmol.L-1)处理DU145细胞24h后Bcl-2和Bax基因的表达。结果 Andro(5.0μmol.L-1)作用24h后,形态学改变可见细胞间开始失去连接,细胞变圆、体积缩小,数量明显减少;不同浓度Andro组DU145细胞生长受到明显抑制,且呈浓度和时间依赖性;经10、20、40μmol.L-1 Andro作用24h后,各组细胞凋亡率分别为(15.19±0.76)%、(29.37±1.75)%和(38.41±1.31)%,与空白对照组的(3.40±0.21)%比较,差异有统计学意义(P<0.05);10、20和40μmol.L-1 Andro组Bcl-2蛋白的表达强度分别为0.82±0.13、0.54±0.17、0.36±0.12,Bax蛋白的表达强度分别为0.69±0.06、0.74±0.11、0.93±0.18,随药物浓度增加Bcl-2的表达逐渐降低,而Bax的表达逐渐升高。结论 Andro可以抑制前列腺癌DU145细胞的体外增殖并促进其凋亡,其作用机制可能与下调Bcl-2和上调Bax有关。Objective To study the anti-tumor effects of andrographolide on human prostate cancer DU145 cells,and to investigate its mechanism of action. Methods Morphologic changes in DU145 cells were observed under inverted microscope before and after treatment with andrographolide（5.0 μmol·L-1） for 24 hours.The proliferation of DU145 cells was measured by MTT after treatment with andrographolide（5.0,10,20,40 and 80 μmol·L-1） for 24,48 and 72 hours.The apoptosis of DU145 cells was detected by flow cytometry using Annexin V-FITC/PI staining and the expression of Bcl-2 and Bax was determined by Western blotting after treatment with andrographolide（10,20 and 40 μmol·L-1） for 24 hours. Results After treatment with andrographolide（5.0 μmol·L-1） for 24 hours,morphological analysis showed round cell shape,reduction in cell volume,decrease in cell number,and disappearance of cell connection.The growth of DU145 cells was inhibited by andrographolide in a dose-and time-dependent manner.Compared with control group,apoptosis rates significantly increased after treatment with andrographolide at concentrations of 10,20 and 40 μmol·L-1for 24 hours [（15.19±0.76）%,（29.37±1.75）% and（38.41±1.31）% vs（3.40±0.21）%,respectively;P＆lt;0.05].The expression intensity was 0.82±0.13,0.54±0.17 and 0.36±0.12 for Bcl-2 and 0.69±0.06,0.74±0.11 and 0.93±0.18 for Bax after treatment with andrographolide at concentrations of 10,20 and 40 μmol·L-1,respectively. Conclusion Andrographolide can inhibit the proliferation and induce the apoptosis in human prostate cancer DU145 cells in vitro through down-regulating Bcl-2 expression and up-regulating Bax expression.

关 键 词：穿心莲内酯 前列腺癌 BCL-2 BAX 体外研究 

分 类 号：R737.25[医药卫生—肿瘤]