泌盐植物长叶红砂质膜Na＋/H＋逆向转运蛋白基因(RtSOS1)全长cDNA的克隆及序列分析  

Cloning and Sequence Analysis of the Plasma Membrane Na^+/H^+Antiporter cDNA in Recretohalophyte Reaumuria trigyna Maxim.

作　　者：党振华[1] 郑琳琳[1] 冯智[1] 王迎春[1] 

机构地区：[1]内蒙古大学生命科学学院,内蒙古自治区牧草与特色作物生物技术重点实验室,内蒙古呼和浩特010021

出　　处：《华北农学报》2013年第3期1-6,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金主任基金项目(31140020);内蒙古自然科学基金重大项目(2012ZD05)

摘　　要：长叶红砂为内蒙古东阿拉善-西鄂尔多斯地区特有珍稀泌盐,强旱生小灌木,对盐渍荒漠环境具有极强适应性。利用RT-PCR和RACE技术从该植物中分离出质膜Na+/H+逆向转运蛋白基因(RtSOS1),该cDNA全长为3 829bp,开放阅读框为3 438 bp,编码一个含1 145个氨基酸的蛋白质,推测分子量为126.76 kDa。氨基酸序列的生物信息学分析推测,该蛋白N端含有11个跨膜结构域,C端为一个长约700个氨基酸的亲水性尾,具有磷酸化和自我抑制结构域。同源性比对和系统发育分析证实,RtSOS1与其他植物的质膜Na+/H+逆向转运蛋白亲缘关系较近。Reaumuria trigyna Maxim.is an endangered small shrub with the features of a recretohalophyte.This species is endemic to the Eastern Alxa Western Ordos area and developed distinctive strategies to adapt to the semi-desert and salty soil environment.A full-length cDNA of the plasma Na＋/H＋antiporter（RtSOS1） was isolated from this species by using RT-PCR and RACE technologies.The 3 829 bp sequence comprised a 3 438 bp open reading frame,encoding an 1 145 amino acids protein with the molecular weight of 126.76 kDa.Bioinformatics analyze reveals that RtSOS1 composed of 11 transmembrane domains within its N terminal portion,and a hydrophilic cytoplasmic tail with the length approximately 700 amino acids in its C-terminal portion.In the C-terminal region,the phosphorylation domain and the auto-inhibited domain are found.The Homology comparison and phylogenetic analysis showed that RtSOS1 is related to plasma membrane Na＋/H＋antiporter in other plant species.

关 键 词：长叶红砂 质膜Na+ H+逆向转运蛋白基因 全长CDNA克隆 

分 类 号：Q78[生物学—分子生物学]