源自合肥地区表观健康鸡的沙门氏菌耐药性与血清型、基因型相关性分析  

Analysis of antimicrobial resistance on Salmonella isolated from nonsymptomatic chickens in clinic in Hefei and its serotype and genotype

作　　者：王元兰[1] 刘军军[1] 邵颖[1] 方艳红[1] 魏建忠[1] 王桂军[1] 孙裴[1] 李郁[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2013年第4期540-546,共7页Journal of Anhui Agricultural University

基　　金：国家科技支撑项目(2009BADB9B01);安徽省高等学校省级自然科学研究项目(KJ2008B056);安徽省家禽产业体系基金

摘　　要：为了解源自合肥地区表观健康鸡的沙门氏菌耐药性及其与血清型、基因型的相关性,在前期已鉴定血清型和ERIC基因型的基础上,采用K-B纸片法对21株沙门氏菌分离株进行21种抗生素药物敏感试验,利用PCR技术对耐药菌株进行32种耐药基因检测。结果显示,总耐药率为100%(21/21),耐2种以上抗生素的菌株占76.19%(16/21),多重耐药谱为青霉素-氨苄西林-阿莫西林-链霉素-复方新诺明,其中耐受β-内酰胺类抗生素的类别最多,耐药率最高达100%(21/21)。21株沙门氏菌均检出耐药基因,涉及blaPSE、sul1、strA、blaTEM、sul2、tetA、tetB、aph3-Ⅱa和catⅠ9种,其中blaPSE基因的检出率最高,为85.7%(8/21)。可见源自合肥地区表观健康鸡的沙门氏菌对β-内酰胺类抗生素耐药性最强,与其携带blaPSE基因有关;耐药表型测定结果与耐药基因检测结果基本一致;耐药性与具有相同来源的同一血清型、基因型相关。In order to investigate the relationship between antimicrobial resistance and serotypes,genotypes of Salmonella isolates from apparently healthy chickens in Hefei,the Kirby-Bauer method was applied to determine the sensitivity of 21 strains susceptibility to 21 antibiotics,and PCR technique also was used to detect 32 kinds of drug-resistance genes,based on identified serotypes and ERIC-PCR gene-type.It was found that 100%（21/21）of the Salmonella isolates were resistant to all antibiotics by Drug Susceptibility Test;76.19%（16/21） were resistant to at least two antimicrobials among the 21 isolates,and the dominating multidrug-resistant spectrum was Penicillin G-Ampicillin-Amoxicillin-Streptomycin-Trimethoprim-sulfamethoxazole.It was the most extensive in resistance to β-lactamase antibiotics by 100%（21/21）.Meanwhile,the resistance genes could be detected from all these isolates by PCR,including blaPSE,sul1,strA,blaTEM,sul2,tetA,tetB,aph3-Ⅱa and catⅠ,and blaPSE gene was the most prevalent with the detected rate of 85.7%（8/21）.It is concluded that the stronggest antimicrobial resistance to β-lactamases drugs on Salmonella isolated from apparently healthy chickens in Hefei due to the existence of blaPSE gene;antimicrobial phenotype is also related to the antimicrobial resistance genes;antimicrobial resistance is in relevant with the same sources of the same serotypes and genotypes.

关 键 词：表观健康鸡 沙门氏菌 耐药性 血清型 ERIC基因型 

分 类 号：S858.31[农业科学—临床兽医学]