蹄叶炎奶牛血浆蛋白质组学2-DE图谱的构建及分析  

Two-DE map analysis of plasma proteomics in dairy cows suffered from laminitis

作　　者：高昭辉[1,2,3] 荔霞[1] 严作廷[1] 王旭荣[1] 阎萍[1,2] 董书伟[1,2] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室/甘肃省中兽药工程技术中心,甘肃兰州730000 [2]甘肃省牦牛繁育重点实验室,甘肃兰州730000 [3]甘肃农业大学动物医学院,甘肃兰州730050

出　　处：《中国兽医学报》2013年第7期1078-1082,共5页Chinese Journal of Veterinary Science

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(BRF100301);国家现代农业产业技术体系建设专项资金资助项目(CARS-40-30)

摘　　要：采集患病牛和健康牛血浆,用人白蛋白和IgG抗体去除其高丰度蛋白后,通过双向凝脉电泳(Two dimension-al gel electrophoresis,2-DE)技术和PDQuest分析软件比较2组间血浆蛋白组表达差异。结果显示,蹄叶炎奶牛血浆2-DE图谱中有39个蛋白斑点表达上调,23个蛋白斑点表达下调,共检测到62个差异蛋白点。结果表明,奶牛发生蹄叶炎时,其血浆蛋白组表达模式发生了改变,这种改变可能与蹄叶炎的发病机制密切相关。In order samples from six to study on differential plasma proteomics of laminitis in dairy cows, plasma sick and healthy cows each were collected. After removed the high abundance proteins in plasma with the antibody of albumin and IgG for human, differential plasma pro- teomics was compared and analyzed between laminitis and healthy cows by 2-DE technology and PDQuest software. The results showed that the total 62 protein spots were significantly different- ly expressed,of which 39 spots were up-regulation and 23 spots were down-regulation. It was in- dicated that the expression pattern of plasma proteomics was changed when laminitis occurred, which was closely related with the mechanism of laminitis. The paper was the first time to re- search on laminitis in dairy cows by proteomics, and the results will establish the foundation for clarifying the mechanism at protein level and screening drug targets for laminitis.

关 键 词：蹄叶炎 蛋白质组学 2-DE 血浆 奶牛 

分 类 号：S852.23[农业科学—基础兽医学] S857.26[农业科学—兽医学]