采用5.0L全自动发酵罐进行钝顶螺旋藻培养的研究  

The research of S.platensis cultivated using a 5.0L automatic fermenter

作　　者：谯顺彬[1] 董汝晶[1] 田辉 张义明[1,3] 陶希芹 罗芳[1] 

机构地区：[1]贵州工业职业技术学院,贵州贵阳550008 [2]贵州茅台酒厂(集团)习酒有限责任公司,贵州习水564622 [3]贵州省发酵工程与生物制药重点实验室,贵州贵阳550003

出　　处：《食品工业科技》2013年第15期144-148,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(39860004);贵州省科学技术基金项目(黔科合)J字【2010】2068号)

摘　　要：采用5.0L全自动机械搅拌式发酵罐作为培养装置,分别进行了钝顶螺旋藻光合自养和混合营养培养研究。通过测定最终藻体细胞中藻体干重(DW)、藻胆蛋白和叶绿素a等成分,可知混合营养条件下,藻体干重比光合自养培养时提高104.75%,但藻胆蛋白和叶绿素a的含量分别降低13.5%和18.2%。在此基础上,进行了螺旋藻混合营养条件下半连续培养研究,结果表明藻体干重比采用混合营养分批培养时提高38.05%,而藻胆蛋白和叶绿素a的含量则分别降低3.76%和1.75%。从最终培养结果可知,利用光生物反应器对螺旋藻进行规模化半连续培养是一种可行的培养方式。A 5.0L automatic fermenter, respectively was used to cultivate the S.pfatensis in the condition of photoautotrophy and mixotrophy,respectively.Firstly,three critical ingredients were measured timely in the process of the cultivating S.platensis under the photoautotrophy and mixotrophy, including dry weight （DW）, phycobiliproteins and chlorophyll-a.According to the experiment results,the DW that was in mixotrophy condition was more ]04.？5% than the photoautotrophy,the phycobiliproteins and the chlorophyll-a yet were lower,the value were 13.5% and 18.2%, respectively.The results showed that the DW was more 38.05% than batch cultivation. However,the phycobiliproteins and the chlorophyll- a yet were lower, the value were 3.？6% and 1.75%, respectively. Therefore, the method which cultivated S.platensis in the condition of mixotrophy using the semi- continuous cultivation mode was a worthy method in photo bioreactor when S.platensis was cultivated in the large- scale.

关 键 词：全自动发酵罐 钝顶螺旋藻 藻体干重(DW) 半连续培养 

分 类 号：Q939.97[生物学—微生物学]