^(60)COγ射线照射大鼠涎腺组织后对Rad50蛋白表达的影响  

Effect of ^(60)COγ irradiation on Rad50 protein expression in rat salivary glands

作　　者：尹恒山[1] 王代友[1] 陈超梅[1] 麦华明[1] 

机构地区：[1]广西医科大学附属口腔医院口腔颌面外科,南宁市530021

出　　处：《实用医学杂志》2013年第14期2257-2258,共2页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:30960420);广西医疗卫生重点科研课题(编号:桂卫重2010082)

摘　　要：目的:初步研究不同剂量60COγ射线照射大鼠涎腺组织后Rad50蛋白的表达变化情况。方法:选取Wistar大鼠60只,随机分成6组:可分为对照组(0Gy组),3、6、9、12、15Gy组,每组10只。用60COγ射线一次性照射大鼠的头颈部后,2h内处死大鼠,并收集大鼠腮腺及颌下腺组织。使用免疫印迹法检测Rad50蛋白在大鼠腮腺、颌下腺的表达情况。结果:放射后大鼠的腮腺及颌下腺组织中的Rad50蛋白的表达量随着放射剂量的不同而变化,并且分别在9Gy及12Gy时达到最高。结论:Rad50蛋白的表达量受放射剂量和组织类型的影响,可能参与了涎腺放射损伤修复的机制。Objective To investigate the effect of different doses of ^60 COγ-ray irradiation on Rad50 protein expression in rat salivary glands.Method Sixty wistar rats were randomly divided into 6 groups：the control group（0 Gy） and 5 experimental groups.The experimental groups were received 3,6,9,12,15 Gy doses of ^60 COγ-ray irradiation,respectively.Western Blot assay was used to detect Rad50 protein expression in rat salivary glands after 2-hour irradiation.Result Rad50 protein expression changed with different doses of irradiation,and Rad50 protein was highly expressed in the group of dose of 9 Gy in parotid gland and in the group of dose of 12 Gy in the submandibular gland.Conclusion Rad50 protein expression was modulated by the radiation dose and the tissue types,and it may be involved in the process of injury and repair of salivary gland after irradiation.

关 键 词：Rad50蛋白 MRN复合体 免疫印迹 涎腺组织 

分 类 号：R739.8[医药卫生—肿瘤]