FMR1基因敲除雄鼠生殖功能下降的机制探讨  

Investigation on the mechanism of descended reproductive function in FMR1 gene knockout male mice

作　　者：叶球仙 杨洁[1] 罗虹[1] 陈盛强[2] 黄晓虹[1] 甘婷[1] 陈燕[1] 肖国宏[1] 

机构地区：[1]广州医学院第三附属医院生殖医学中心,510150 [2]广州医学院第二附属医院神经科学研究所,510260

出　　处：《实用医学杂志》2013年第14期2262-2264,共3页The Journal of Practical Medicine

基　　金：广东省教育部产学研结合项目(编号:2008B090500258)

摘　　要：目的:利用FMR1基因敲除鼠(KO)和野生型FVB鼠(WT),研究FMR1基因敲除引起雄鼠生殖功能下降的可能机制。方法:随机取10周的KO及WT雄鼠,HE染色观察睾丸形态;免疫组化及ImageProPlus6.0测定分析下丘脑神经肽Y(NPY)及γ-氨基丁酸(GABA)表达的平均光密度值;ELISA测定血清NPY水平。结果:KO、WT小鼠睾丸形态未见明显不同;KO雄鼠下丘脑NPY的表达弱于WT雄鼠(0.27±0.031,0.31±0.031,P<0.05);KO雄鼠血清NPY的表达弱于WT雄鼠(179.21±50.773,225.24±52.293,P<0.05);两种基因型小鼠下丘脑GABA的表达差异无统计学意义(0.29±0.017,0.28±0.009,P>0.05)。结论:FMR1基因可能是通过下调NPY的表达来降低雄鼠生殖功能的。Objective To investigate the mechanism of descended reproductive function induced by FMR1 gene knockout in male FMR1 gene knockout mice（KO） and male wild mice（WT）.Methods The morphology of the testis of 10 weeks old KO and WT male mice were observed by HE staining.The mean optical density（MOD） of the expressions of neuropeptide Y（NPY） and gamma aminobutyricacid（GABA） in hypothalamus were tested and analyzed by immunohistochemistry and Image Pro Plus 6.0.Serum NPY concentrations were measured by ELISA.Results No obvious pathological differences in testis were found between KO and WT mice.The MOD of NPY in hypothalamus and the expression of NPY in serum in KO mice were weaken than those in WT mice,respectively [0.27 ± 0.031 vs.0.31 ± 0.031,P〈0.05;（179.21 ± 50.773）ng /L vs.（225.24 ± 52.293） ng /L,P〈0.05].There was no significant difference in the MOD of GABA in hypothalamus between KO and WT mice（0.29 ± 0.017 vs.0.28 ± 0.009,P〈0.05）.Conclusions FMR1 gene may down-regulate the reproductive function of male mice through reducing the expression of NPY.

关 键 词：脆性X智力低下基因1 神经肽Y Γ-氨基丁酸 生殖 

分 类 号：R-332[医药卫生]