参附注射液对大鼠心肺复苏后脑缺血损伤保护作用的研究  

Protective Effect of Shenfu Injection on Cerebral Injury after Cardiopulmonary Resuscitation in Rats

作　　者：章杰[1] 陈寿权[1] 李章平[1] 程俊彦[1] 黄唯佳[1] 

机构地区：[1]温州医学院附属第一医院急诊科,浙江温州325003

出　　处：《中华中医药学刊》2013年第7期1631-1634,I0010,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药青年基金资助项目(2005Y018)

摘　　要：目的:探讨参附注射液对大鼠心肺复苏后血清S100β蛋白、神经元特异性烯醇化酶和脑组织病理损伤的影响。方法:成年雄性SD大鼠160只,随机分为对照组、复苏组、小剂量参附组(10 mL/kg)和大剂量参附组(20 mL/kg),每组40只。建立窒息型大鼠心肺复苏模型,分别于各时间点(0.5 h、3 h、6 h、12 h和24 h)取血取样,ELISA法测定血清S100β蛋白、神经元特异性烯醇化酶(NSE)水平,光镜和电镜下观察大脑皮层组织病理改变。结果:复苏组和大、小剂量参附组血清S100β蛋白、NSE水平在ROSC后各个时间点均显著高于对照组(P<0.05或P<0.01);大、小剂量参附组与复苏组同时间比较,从ROSC后6h起血清S100β蛋白含量均显著降低(P<0.05或P<0.01);同复苏组相比,小剂量参附组ROSC后6h、12h血清NSE含量显著降低(P<0.05),大剂量参附组从ROSC后6h开始,血清NSE浓度显著降低(P<0.01);在ROSC后各个时相大、小剂量参附组间相比,血清S100β蛋白、NSE含量均无显著性差异(P>0.05)。光镜和电镜下组织病理改变均显示,大小剂量参附组大脑皮层组织结构损害程度明显轻于复苏组。结论:参附注射液尤其是大剂量应用对大鼠心肺复苏后脑缺血损伤具有较明显保护作用。Objective：To investigate the serum S100β protein and neuron-specific enolase（NSE） levels and the histopathological change of cerebral cortex in asphyxia rats following Shenfu Injection infusion after cardiopulmonary resuscitation（CPR）.Methods：160 male adult SD rats were randomly divided into 4 groups： sham-operation group,CPR group,low-dose Shenfu Injection group and high-dose Shenfu Injection group.And each group was further divided into 5 subgroups respectively on the base of time（0.5 h,3 h,6 h,12 h,24 h after incision of trachea or ROSC,n=8）.Asphyxiation cardiac arrest model was used and samples were taken on each time point of subgroups.Serum levels of S100β protein and NSE were measured with enzyme-linked immunosorbent assay（ELISA）respectively.The pathological change of cerebral cortex was observed under optical microscope and electron microscope.Results：The serum levels of S100β protein and NSE were elevated significantly in CPR group,low-dose and large-dose Shenfu Injection groups compared with sham-operation group（P〈0.05 or P〈0.01）.Serum S100β protein level started to decrease at 6 h after ROSC both in low-dose and large-dose Shenfu Injection groups compared with CPR group（P〈0.05 or P〈0.01）.Serum NSE level of low-dose Shenfu Injection group decreased at 6 h and 12 h after ROSC compared with CPR group（P〈0.05）.Serum NSE level of large-dose Shenfu Injection group started to decrease at 6 h after ROSC compared with CPR group（P〈0.01）.There was no significant difference at serum levels of S100β protein and NSE between low-dose and large-dose Shenfu Injection groups on each time-point after ROSC.Optical microscopy and electron microscope revealed histopathological changes of cerebral cortex were much more minor in low-dose and large-dose Shenfu Injection groups than those in CPR group.Conclusion：Shenfu Injection,especially at large dose,could alleviate brain ischemic injury after cardiopulmonary resuscitation.

关 键 词：心肺复苏 S100Β蛋白 神经元特异性烯醇化酶 参附注射液 

分 类 号：R285.5[医药卫生—中药学]