左乙拉西坦对癫大鼠海马组织中SYN和GAP-43mRNA表达的影响  

Effects of levetiracetam on the expression of SYN and GAP-43mRNA in the hippocampus of rats with epilepsy

作　　者：张岩[1] 王海燕[1] 王影[1] 王蕾[1] 聂磊[1] 

机构地区：[1]佳木斯大学第一附属医院儿内科,黑龙江佳木斯154007

出　　处：《临床儿科杂志》2013年第7期670-673,共4页Journal of Clinical Pediatrics

摘　　要：目的观察左乙拉西坦(LEV)对癫大鼠空间学习记忆能力及海马组织中突触素(SYN)和神经生长相关蛋白-43(GAP-43)表达的影响。方法 SD大鼠随机分为正常对照组,癫模型组,癫模型LEV干预组(LEV组),LEV对照组。通过氯化锂–匹罗卡品诱导建立癫大鼠模型。于建模成功后24 h,LEV组和LEV对照组给予LEV 150 mg/(kg d);正常对照组和癫模型组给予等量生理盐水。于建模成功后的第7、14、28天通过RT-PCR方法观察各组大鼠海马组织中SYN和GAP-43 mRNA的表达水平,并于第4周应用Morris水迷宫评价大鼠的学习记忆能力。结果 Morris水迷宫测试中,LEV对照组与正常对照组相比,逃避潜伏期和穿越平台次数的差异无统计学意义;癫模型组的逃避潜伏期延长、穿越平台次数减少,与正常对照组比较差异有统计学意义(P<0.05);而LEV组与癫模型组相比,逃避潜伏期缩短、穿越平台次数增加,差异有统计学意义(P<0.05)。LEV对照组与正常对照组相比,SYN和GAP-43 mRNA表达量的差异无统计学意义;癫模型组的SYN和GAP-43 mRNA的表达量明显降低,与正常对照组比较差异有统计学意义(P<0.05);LEV组与癫模型组相比,SYN和GAP-43 mRNA的表达量明显增高,差异有统计学意义(P<0.05)。结论 LEV可改善癫大鼠的学习记忆能力,该作用可能与其调节海马组织中SYN和GAP-43 mRNA的表达有关。Abstract： Objectives To observe the effects of levetiracetam （LEV） on spatial learning and memory ability in epileptic rats and the expression of synaptophysin （SYN） and growth-associated protein 43 （GAP-43） in the hippocampus of rats with epi- lepsy induced by lithium piloearpine （Li-pilo）. Methods SD rats were randomly divided into normal control group （normal saline group）, LEV group （treatment group）, Li-pilo group （model group） and LEV control group （normal dose group）. Twenty-four hours after the establishment of model, LEV （150 mg/kg per day） was given to rats in LEV group and LEV control group, and equivalent normal saline was given to rats in normal control group and Li-pilo group. At 7, 14 and 28 days after successful model building, the expression levels of the SYN and GAP-43mRNA in hippocampus of rats in each group were detected by RT-PCR. In the 4th week, the ability of learning and memory of rats in each group was evaluated by the Morris water maze. Results There was no significant difference in escape latency and the frequency of crossing platform between LEV control group and normal control group in Morris water maze test. Compared with normal control group, the rats in Li-pilo group had a longer latency to escape and reduced frequency of crossing platform （P〈0.05）. Compared with Li-pilo group, the rats in LEV group had shorter latency to escape and more frequency of crossing platform （P〈0.05）. There was no significant difference in the expressions of SYN and GAP-43mRNA in hippocampus of rats between LEV control group and normal control group. The expressions of SYN and GAP- 43mRNA in Li-pilo group were lower than those of normal control group and LEV group （P〈0.05）. Conclusions Levetiracetam may improve learning and memory ability of rats with epilepsy through the regulation of SYN and GAP-43mRNA expressions inhippocampal tissue.

关 键 词：左乙拉西坦 癫 突触素 神经生长相关蛋白-43 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]