RNA干扰TGF-β信号通路对人膀胱癌T24细胞迁移和侵袭能力的影响  

Inhibition of TGF-β by siRNA Suppresses the Motility and Invasiveness of T24 Bladder Cancer Cells

作　　者：李玉兵[1,2] 江少波[1,2] 任小刚[1,2] 孙洁[1,2] 谢立平[3] 

机构地区：[1]浙江省中医院 [2]浙江中医药大学附属第一医院泌尿外科,杭州310006 [3]浙江大学医学院附属第一医院泌尿外科,杭州310003

出　　处：《医学研究杂志》2013年第7期150-154,共5页Journal of Medical Research

摘　　要：目的探讨过表达的TGF-β1和作用于TGF-β受体Ⅰ的干扰RNA(TsiRNA)在体外对人膀胱癌T24细胞迁移、侵袭能力的影响。方法采用Transwell迁移试验、划痕试验以及Transwell侵袭实验观察过表达的TGF-β1和TsiRNA在体外对人膀胱癌T24细胞迁移、侵袭能力的影响,RT-PCR技术和蛋白免疫印迹法(Western blot)检测TGF-β1及TsiRNA处理后TGF-β受体Ⅰ基因与蛋白表达水平的变化。结果过表达的TGF-β1可以显著提高膀胱癌T24细胞的迁移、侵袭能力,而TsiRNA可以完全降低由TGF-β1引起的T24的迁移、侵袭能力的变化。结论膀胱癌细胞的迁移、侵袭能力与过表达的TGF-β1密切相关,TsiRNA可以降低T24细胞的迁移、侵袭能力。Objective To evaluate the effects of TGF - β1 and TsiRNA targets the TGF - β type Ⅰ receptor on the motility and in- vasiveness of T24 cell. Methods We blocked the TGF - β signal pathway in T24 human bladder cancer cells with a siRNA （TsiRNA） which targets the TGF - β typeⅠreceptor and evaluated the effects of TGF - β1 and TsiRNA on the cell motility and invasiveness by Ma- trigel migration assay, wound -healing assay and Matrigel invasion assay. Western blot and RT- PCR analysis were used to examine the effects of TGF - β1 and TsiRNA on the expression of TGFBRI. Results Our study revealed an addition of TGF - β1 （2 ng/ml） signifi- cantly enhanced the migration and migration of the T24, while this enhanced ability could be effectively inhibited by TsiRNA. Conclusion This study suggested that TGF - β1 was a major enhancer for the motility and invasiveness of bladder cancer cells. The TGFBRI targeted siRNA strongly inhibited the motility and invasiveness of the bladder cancer cells.

关 键 词：膀胱癌 转化生长因子Β1 转化生长因子β受体Ⅰ RNA干扰 细胞迁移 侵袭 

分 类 号：R730.21[医药卫生—肿瘤]