miR495、miR551a靶向干扰PRL-3表达抑制胃癌腹膜转移  

MiR495-and miR551a-mediated down-regulation of PRL-3 expression inhibits peritoneal metastasis in a mouse model of gastric cancer

作　　者：许牧[1,2] 曹毅[2] 蒋蒙蒙[1,2] 揭志刚[2] 李正荣[2] 

机构地区：[1]南昌大学研究生院医学部 [2]南昌大学第一附属医院胃肠外科,江西省南昌市330006

出　　处：《世界华人消化杂志》2013年第18期1693-1700,共8页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30901429~~

摘　　要：目的:研究miR495、miR551a干扰质粒对SGC7901胃癌细胞中促肝细胞再生磷酸酶-3(phosphatase of regenerating liver-3,PRL-3)表达的影响,探讨基因干扰在胃癌治疗中的价值.方法:分别体外培养稳定转染后的SGC7901细胞系及未经处理的SGC7901细胞系,取对数生长期瘤细胞,0.5mL(1×107/mL)细胞悬液接种于Balb/ca(nu/nu)裸鼠腹腔内,在SPF条件下饲养1mo后处死,观察裸鼠成瘤率、瘤体生长情况及腹膜转移等情况,并行组织病理学检查.取各组部分移植瘤行荧光定量PCR检测,对比各组miR495、miR551a及PRL-3mRNA的相对表达水平.结果:各组裸鼠成瘤率均100%,其中两实验组裸鼠一般情况及生存期均好于对照组.取各组移植瘤行荧光定量PCR检测显示,两实验组裸鼠miRNA表达量显著高于对照组,PRL-3mRNA表达量低于对照组.转染质粒组胃癌迁移能力明显减弱.结论:转染靶向干扰PRL-3表达的miR495、mi551a真核质粒可以明显抑制胃癌细胞体内转移侵袭能力.AIM： To investigate the effect of miR495 and miR551 on PRL-3 expression in gastric cancer SGC7901 cells to explore gene interference therapies for gastric cancer. METHODS： SGC7901 cells stably transfected with miR495 or miR551a and non-transfected SGC7901 cells were inoculated intraperitoneally in BALB/CA （nu/nu） nude mice. One month after inoculation, the mice were killed to observe the rate of tumor formation, tumor growth and migration. Real-time PCR was performed to quantify mRNA levels of miR495, miR551a and PRL-3. RESULTS： Compared to nude mice inoculated with non-transfected cells, miR495 and miR551a levels were increased, PRL-3 level was decreased, and migration of gastric cancer was suppressed in mice inoculated with SGC7901 cells stably transfected with miR495 or miR551a. CONCLUSION： MiR495 and miR551a can suppress the migration of gastric cancer by inhibiting PRL-3 expression in mice.

关 键 词：miR495 miR551a 促肝细胞再生磷酸酶-3 胃癌 动物模型 

分 类 号：R735.2[医药卫生—肿瘤]