抑癌基因WWOX与p53在胃癌组织中的表达及相互关系  

Expression of WWOX and p53 in gastric cancer

作　　者：李小华[1] 何利平[2] 罗忠金[1] 

机构地区：[1]厦门市第二医院消化内科,福建省厦门市361021 [2]福建省立医院消化科,福建省福州市350000

出　　处：《世界华人消化杂志》2013年第18期1701-1707,共7页World Chinese Journal of Digestology

摘　　要：目的:研究抑癌基因WWOX与p53在胃癌(gastric cancer,GC)中的表达及相互关系,探讨其在GC发生、发展中可能的机制.方法:随机选择手术切除的GC石蜡标本70例及相应癌旁正常组织的石蜡标本20例,采用免疫组织化学的方法检测WWOX、p53蛋白表达.对所得数据进行统计学分析.结果:p53蛋白在G C中的表达率为51.42%(36/70),癌旁正常组织的表达率为0.00%(0/20);淋巴结转移组表达显著高于无转移组(2=4.79,P<0.05);晚期GC组表达高于早期GC(2=6.62,P<0.05).WWOX蛋白在GC中的表达率为41.43%(29/70),癌旁正常组织的表达率为90.00%(18/20);淋巴结转移组与无转移组相比,无统计学意义(2=0.29,P>0.05);晚期GC显著低于早期GC(2=9.36,P<0.05).WWOX与p53表达呈负相关(r=-0.61,P<0.05).结论:p53、WWOX蛋白在GC中表达,与浸润深度、淋巴结转移、临床分期相关,二者在GC发生、发展中相互作用.AIM： To examine the expression of WWOX and p53 proteins in gastric cancer, and to analyze the possible mechanisms behind the malignant growth of gastric cancer. METHODS： The expression of WWOX and p53 proteins was detected by immunohistochemistry in 70 paraffin-embedded samples of gastric cancer and 20 tumor-adjacent normal tissue samples. Statistical analyses were then performed to analyze the relationship between WWOX and p53 expression and clinicopathologic parameters of gastric cancer. RESULTS： The positive rate of expression of p53 protein in gastric cancer was significantly higher than that in normal gastric tissue （51.42% vs 0.00%, P 0.05）. Expression of p53 protein in gastric cancer was related with depth of invasion, lymph node metastasis, and tumor clinical stage （all P 0.05）. The positive rate of expression of WWOX protein was significantly lower in gastric cancer than in normal gastric tissue （41.43% vs 90.00%, P 0.05）. Expression of WWOX protein in gastric cancer was related with depth of invasion and clinical stage, but not with lymph node metastasis. There is a negative correlation between WWOX and p53 expression in gastric cancer. CONCLUSION： Detection of expression of p53 protein and WWOX proteins might be useful for early diagnosis and evaluation of prognosis of gastric cancer.

关 键 词：胃癌 WWOX P53 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]