SLP-2 siRNA对裸鼠胃癌移植瘤细胞增殖及凋亡的影响  

Effect of siRNA-mediated SLP-2 silencing on tumor cell proliferation and apoptosis in nude mice bearing gastric tumor xenografts

作　　者：张剑[1] 吴敏[1] 王丽娟[1] 张红巧[1] 师广勇[1] 巴楠[1] 张自森[1] 闫琳[1] 郑晓珂[1] 

机构地区：[1]郑州大学第五附属医院肿瘤科,河南省郑州市450052

出　　处：《世界华人消化杂志》2013年第18期1740-1744,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨SLP-2siRNA对裸鼠胃癌移植瘤细胞增殖及凋亡的影响.方法:设计合成化学修饰的SLP-2siRNA,将胃癌细胞SGC-7901接种于裸鼠皮下建立裸鼠胃癌移植瘤模型,随机分为转染SLP-2siRNA组、阴性对照组和空白对照组,于肿瘤局部分别多点多次注射化学修饰SLP-2siRNA、阴性对照siRNA及生理盐水.检测3组移植瘤细胞增殖及凋亡状况,RT-PCR及免疫组织化学技术检测移植瘤细胞内SLP-2mRNA及蛋白的表达.结果:与两对照组相比,注射化学修饰的SLP-2siRNA后,裸鼠胃癌移植瘤细胞生长速度减慢、移植瘤体积减小(P=0.009,P=0.003).抑制瘤率分别为26.74%和30.15%,细胞凋亡无明显变化(P>0.05),肿瘤细胞内SLP-2mRNA及蛋白的表达量降低.结论:SLP-2siRNA可抑制人胃癌裸鼠移植瘤的生长,但对细胞凋亡无影响,提示SLP-2基因参与促进胃癌细胞增殖.AIM： To investigate the effect of siRNA-mediated SLP-2 silencing on tumor cell proliferation and apoptosis in nude mice bearing gastric tumor xenografts. METHODS： Chemically modified SLP-2 siRNA was designed and constructed. A tumor-bearing model was developed by inoculation of gastric cancer SGC-7901 cells into BALB/c nude mice subcutaneously. All mice were randomized into three groups： a SLP-2 siRNA-transfected group, a negative control group and a blank control group. Chemically modified SLP-2 siRNA and a negative control siRNA were injected into tumor xenografts of the SLP-2 siRNA transfected group and negative control group, respectively,while the blank control group only received an injection of equal volume of saline. Tumor volume was recorded and the apoptosis index was observed. The expression of SLP-2 mRNA and protein in tumor tissue was measured by RTPCR and immunohistochemistry. RESULTS： Compared to the two control groups, tumor volume was significantly decreased in the SLP-2 siRNA transfected group （P = 0.009, 0.003）, and the reduced rate of tumor growth was 26.74% and 30.15%. The number of apoptotic cells and apoptosis index showed no significant differences between the SLP-2 siRNA transfected group and the two control groups （both P 0.05）. CONCLUSION： SiRNA-mediated SLP-2 silencing inhibits tumor cell growth but has no significant effect on tumor cell apoptosis in tumor xenografts.

关 键 词：胃癌 SLP-2 SIRNA 裸鼠移植瘤模型 

分 类 号：R735.2[医药卫生—肿瘤]