胃癌患者TYMS、ERCC1基因型在外周血和肿瘤组织中的多态性  

Comparison of TYMS and ERCC1 genotypes between peripheral venous blood and tumor tissues of patients with gastric cancer

作　　者：王珊珊[1] 沈燕[1] 李慧莲[2] 卫肖[1] 

机构地区：[1]郑州大学第一附属医院,河南省郑州市450052 [2]河南省军区门诊部,河南省郑州市450000

出　　处：《世界华人消化杂志》2013年第18期1760-1765,共6页World Chinese Journal of Digestology

摘　　要：目的:检测胃癌(gastric cancer,GC)患者外周血和肿瘤组织中胸苷酸合成酶基因(thymidylatesynthase,TYMS)和切除修复交叉互补基因1(excision repair cross-complementing 1,ERCC1)的多态性,比较各基因型在外周血和肿瘤组织中是否一致.方法:收集43例GC患者术前抗凝外周血及肿瘤组织,将其分为外周血组与肿瘤组织组.应用PCR法和限制性片段长度多态性聚合酶链式反应(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)法分别检测外周血与肿瘤组织中TYMS和ERCC1基因的基因型.结果:TYMS和ERCC1基因各基因型与GC患者临床病理因素无显著相关(P>0.05).外周血与肿瘤组织中TYMS基因3R/3R、(2R/2R、2R/3R)基因型检出率76.7%、23.3%,ERCC1基因C/C、(T/T、C/T)基因型检出率为81.3%、18.7%,差别有统计学意义(P<0.01).结论:GC患者外周血和肿瘤组织中TYMS与ERCC1基因各基因型检出率一致,提示外周血标本可代替肿瘤组织标本对TYMS与ERCC1基因多态性进行检测.AIM： To compare the genotypes of thymidylatesynthase （TYMS） and excision repair crosscomplementing 1 （ERCC1） between peripheral venous blood and tumor tissues of patients with gastric cancer （GC）. METHODS： Peripheral venous blood and tumor tissue samples of 43 GC patients were collected. The genotypes of TYMS and ERCC1 in these samples were detected by PCR and polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. RESULTS： There was no significant correlation between TYMS and ERCC1 genotypes and clinicopathologic parameters in GC patients （all P 0.05）. In both peripheral blood and tumor tissue samples, the detection rate of 3R/3R genotype and that of 2R/2R or 2R/3R genotype of TYMS were 76.7% and 23.3%, respectively; and the detection rate of C/C genotype and that of T/T or C/T genotype of ERCC1 were 81.3% and 18.7%, respectively. The detection rates of TYMS and ERCC1 genotypes in peripheral blood were consistent with those in tumor tissue samples （P 0.01）. CONCLUSION： Peripheral venous blood samples can be used as an alternative to tumor tissue samples to detect gene polymorphisms.

关 键 词：胃癌 聚合酶链式反应 限制性片段长度多态性聚合酶链式反应 基因多态性 胸苷酸合成酶基因 切除修复交叉互补基因1 

分 类 号：R735.2[医药卫生—肿瘤]