肿瘤相关基因对大肠肿瘤临床诊断及预后应用的研究  

Tumor Related Genes on Clinical Diagnosis and Prognosis Application of Large Intestine Tumors

作　　者：王中易[1] 赵金伟[2] 王欣平[1] 陈玉芹 孙明[4] 

机构地区：[1]吉林省人民医院,吉林长春130021 [2]吉林省武警总队医院,吉林长春130052 [3]北华大学医学院,吉林吉林132001 [4]中国人民解放军五七〇四厂医院,吉林吉林132001

出　　处：《北华大学学报（自然科学版）》2000年第2X期122-124,共3页Journal of Beihua University（Natural Science）

基　　金：吉林省科委资助项目!(970 334 )

摘　　要：目的 探讨Ｒａｓ，Ｐ５３基因对大肠肿瘤的早期诊断及预后的临床应用价值。方法 用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）ＥＢ染色技术对２５例大肠肿瘤原发灶组织进行了Ｋｉ－，Ｎ－ｒａｓ第１处显子和Ｐ５３基因５～８外显子的点突变进行检测。息肉性腺瘤组织中１／２（５０％）发生了Ｋｉ－ｒａｓ点突变。在大肠癌组织中，存在Ｎ－ｒａｓ点突变、Ｋｉ－ｒａｓ点突变、Ｐ５３基因５～８外显子突变。Objective To investigate Ras,P53 genes on clinical diagnosis and prognosis of large intestine tumors.Methods Using a polymerase chain reaction simple strand conformation polymorphism (PCR SSCP) EB staining technique,25 human coloretal tumors for mutation in exons 5,6,7,8 of P53 genes and exon 1 of Ki ,N ras were checked.Results Two polypoid adenoma cases were found Ki ras point mutation positive.In coloretal cancer teste,there were N ras mutation,Ki ras point mutation,and P53 genes mutation positive.Conclusion Ki ras point mutation existed in each stage early diagnosis in coloretal tumors.P53 exons point mutation rate was associated with metastasis of large intestine tumor.

关 键 词：大肠肿瘤 RAS基因 肿瘤相关基因 诊断 预后 

分 类 号：R735.340.4[医药卫生—肿瘤]