不同剂量三七皂苷单体Rg1下调大鼠低O_2高CO_2性肺动脉平滑肌细胞ERK1/2的表达  

ERK1/2 expression of pulmonary arterial smooth muscle cells downregulated by notoginsenoside Rg1 with various concentrations during hypoxia hypercapnia in rats

作　　者：陈丹[1] 郝卯林[1] 何金波[1] 黄林静[1] 马迎春[1] 陈海娥[1] 唐兰兰[2] 王万铁[1] 

机构地区：[1]温州医科大学病理生理学教研室,浙江温州325035 [2]浙江中医药大学附属第二医院病理科,浙江杭州310005

出　　处：《温州医学院学报》2013年第7期444-447,共4页Journal of Wenzhou Medical College

基　　金：浙江省中医药科技计划重点资助项目(2008ZA017);浙江省中医药重点学科建设计划项目(2012-XK-A28)

摘　　要：目的:观察在低O2高CO2条件下,不同剂量的三七皂苷单体Rg1对肺动脉平滑肌细胞(PASMCs)细胞外信号调节激酶(ERK1/2)表达的影响。方法:分离并纯化SD大鼠PASMCs,随机分6组:常氧对照组(N组)、低O2高CO2组(H组)、DMSO对照组(HD组)、Rg1低、中、高剂量干预组(RgL组、RgM组、RgH组)。分别以Western blot和RT-PCR法检测磷酸化ERK(p-ERK)蛋白和ERK1及ERK2 mRNA的表达水平。结果:与N组相比,HD组p-ERK蛋白和ERK1、ERK2 mRNA表达水平明显高于N组(P<0.01);RgL、RgM、RgH组在不同程度上抑制了p-ERK蛋白和ERK1、ERK2 mRNA的表达(P<0.01),其中三七皂苷单体Rg1以40mg/L的浓度为佳。结论:三七皂苷单体Rg1可下调低O2高CO2条件下大鼠PASMCs ERK1/2的表达,这可能是Rg1减轻低O2高CO2性肺动脉收缩(HHPV)的机制之一。Objective： To investigate the effect of notoginsenoside Rg1 with various concentrations on extracellular signal regulated kinase （ERK1/2） signaling pathway in pulmonary arterial smooth muscle cells （PASMCs） under hypoxia and hypercapnia condition. Methods： PASMCs were primarily cultured, and randomly divided into six groups： SD rat under normoxic condition as control group （N group）, hypoxia hypercapnia group （H group）, DMSO control group （HD group）, Rgl-treatment groups （RgL, RgM and RgH group）. Western-blot and RT-PCR were used to determine the expression of p-ERK protein and the expression of ERK1 and ERK2 mRNA, respectively. Results： Western-blot and RT-PCR analysis indicated that the expression of p-ERK protein, ERK1 and ERK2 mRNA were significantly higher in HD group than that in N group （P〈0.01）. Whereas, the level of ERK1/2 mRNA was markedly lower in Rgl-treatment groups than that in HD group （P〈0.01）, and the optimal concentration of Rg 1 is 40 mg/L. Conclusion： Notoginsenoside Rg 1 can attenuate HHPV, which is related to the downregulation of expression of ERK1/2.

关 键 词：肺动脉平滑肌细胞 低O2高CO2 ERK1/2通路 三七皂苷单体Rg1 大鼠 

分 类 号：R363[医药卫生—病理学]