高压氧对荷人喉咽鳞癌小鼠肿瘤生长的影响  

Effects of hyperbaric oxygen on the tumor growth of human pharyngeal squamous cancer in mouse

作　　者：黄天桥[1] 梁大鹏[1] 黄沂传[1] 李慎玲[1] 姜晓丹[1] 李薇[1] 王培嵩[2] 

机构地区：[1]青岛大学医学院附属医院耳鼻咽喉头颈外科,山东省青岛266000 [2]青岛大学医学院附属医院高压氧科,山东省青岛266000

出　　处：《中华航海医学与高气压医学杂志》2013年第3期162-164,173,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的探讨高压氧对荷人喉咽鳞癌小鼠肿瘤生长的影响。方法将32只雄性Balb／c小鼠按数字表法随机分为4组：A组（成瘤5d后处死）、B组（成瘤10d后处死）、C组（成瘤后高压氧暴露5d后处死）、D组（成瘤后高压氧暴露10d后处死），每组8只。接种经消化的对数期人喉咽鳞癌Fadu细胞，小鼠荷瘤后分别暴露于常压空气和高压氧中，各组相应处理后检测其肿瘤质量、体积，进行组织病理学及免疫组织化学分析。结果各组小鼠移植瘤组织病理学结果均为低分化鳞状细胞癌。C组肿瘤质量抑制率为20．13％、体积抑制率为20．54％，成纤维细胞活化蛋白叫FAPα）表达强度明显低于A组（P〈0．05）；D组肿瘤质量抑制率为13．65％、体积抑制率为10．30％，FAPα表达强度明显低于B组（P〈0．05）。结论高压氧对荷人喉咽癌小鼠肿瘤的生长有一定的抑制作用。Objective To explore the effect of hyperbaric oxygen （HBO） on the tumor growth of human pharyngeal cancer in mouse model. Methods Thirty-two male Balb/e mice were randomly divided into 4 groups： group A or the control group （sacrificed 5 days after tumor loading） , group B （sacrificed 10 days after tumor loading ）, group C （sacrificed 5 days after tumor loading and HBO exposure ）, and group D （sacrificed 10 days after tumor loading and HBO exposure ）, each consisting of 8 animals. Following vaccination with the digested pharyngeal cancer Fadu cells in logarithmic phase, the tumor-loaded mice were respectively exposed to atmospheric air and HBO. Then, tumor volume, weight, histopathology and immunohistochemistry were detected for the animals of each group. Results Histopathology of the tumor-loaded mice in each group displayed low-differentiated squamous cell carcinoma. For the animals in group C, tumor mass inhibition rate was 20.13% , and volume inhibition rate was 20.54%. The expression level of fibroblast activation protein-alpha （FAPα） was obviously inferior to that of group A （ P 〈 0.05 ）. For the animals in group D, tumor mass inhibition rate was 13, 65% , and volume inhibition rate was 10.30% and the expression level of FAPα was obviously inferior to that of group B （ P 〈 0.05 ）. Conclusions HBO seemed to have certain effect on the inhibition of tumor growth of human pharyngeal cancer in mice.

关 键 词：高压氧 喉咽癌 小鼠 裸 肿瘤抑制率 

分 类 号：R739.65[医药卫生—肿瘤]